Published online 29 January 2004
Nucleic Acids Research, 2004, Vol. 32, No. 2 590-597
© 2004 Oxford University Press
Sequence-specific transcriptional repression by an MBD2-interacting zinc finger protein MIZF
Department of Biomolecular Sciences, Institute of Biomedical Sciences, Fukushima Medical University School of Medicine, Fukushima 960-1295, Japan
*To whom correspondence should be addressed. Tel: +81 24 547 1660; Fax: +81 24 548 3041; Email: matam{at}fmu.ac.jp
MBD2 is a member of the methyl-CpG-binding protein family that plays an important role in methylated DNA silencing. We have recently identified a novel zinc finger protein, MIZF, as an MBD2-binding partner. To understand the physiological function of MIZF in MBD2-mediated gene silencing, we investigated the DNA-binding properties of MIZF and its potential target genes. Using a cyclic amplification and selection of targets technique, the consensus sequence CGGACGTT, which contains a conserved CGGAC core, was determined as sufficient for MIZF binding. Deletion of individual zinc fingers revealed that five of the seven zinc fingers are required for DNA binding. Reporter assays demonstrated that MIZF represses transcription from the promoter including this DNA sequence. A database search indicated that a variety of human genes, including Rb, contain this sequence in their promoter region. MIZF actually bound to its recognition sequence within the Rb promoter and repressed Rb transcription. These results suggest that MIZF, through its DNA-binding activity, acts as a sequence-specific transcriptional repressor likely involved in MBD2-mediated epigenetic gene silencing.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  H. Donato, N. I. Krupenko, Y. Tsybovsky, and S. A. Krupenko 10-Formyltetrahydrofolate Dehydrogenase Requires a 4'-Phosphopantetheine Prosthetic Group for Catalysis J. Biol. Chem., November 23, 2007; 282(47): 34159 - 34166. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Medina, M. van der Deen, A. Miele-Chamberland, R.-L. Xie, A. J. van Wijnen, J. L. Stein, and G. S. Stein The HiNF-P/p220NPAT Cell Cycle Signaling Pathway Controls Nonhistone Target Genes Cancer Res., November 1, 2007; 67(21): 10334 - 10342. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Hayes, R. P. Ross, G. F. Fitzgerald, C. Hill, and C. Stanton Casein-Derived Antimicrobial Peptides Generated by Lactobacillus acidophilus DPC6026. Appl. Envir. Microbiol., March 1, 2006; 72(3): 2260 - 2264. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Simonsen, L. Sando, D. C. Ireland, M. L. Colgrave, R. Bharathi, U. Goransson, and D. J. Craik A Continent of Plant Defense Peptide Diversity: Cyclotides in Australian Hybanthus (Violaceae) PLANT CELL, November 1, 2005; 17(11): 3176 - 3189. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Auriol, L.-M. Billard, F. Magdinier, and R. Dante Specific binding of the methyl binding domain protein 2 at the BRCA1-NBR2 locus Nucleic Acids Res., July 28, 2005; 33(13): 4243 - 4254. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Kitamura, M. Sekimata, S.-i. Kikuchi, and Y. Homma Involvement of poly(ADP-ribose) polymerase 1 in ERBB2 expression in rheumatoid synovial cells Am J Physiol Cell Physiol, July 1, 2005; 289(1): C82 - C88. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Rao, S. Chopra, G. Ram, A. Gupta, and A. Ranganathan Application of the "Codon-shuffling" Method: SYNTHESIS AND SELECTION OF DE NOVO PROTEINS AS ANTIBACTERIALS J. Biol. Chem., June 24, 2005; 280(25): 23605 - 23614. [Abstract] [Full Text] [PDF]
|
|