Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (278K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (22)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Turan, K.
Right arrow Articles by Nagata, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Turan, K.
Right arrow Articles by Nagata, K.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Published online 29 January 2004

Nucleic Acids Research, 2004, Vol. 32, No. 2 643-652
© 2004 Oxford University Press

Nuclear MxA proteins form a complex with influenza virus NP and inhibit the transcription of the engineered influenza virus genome

Kadir Turan, Masaki Mibayashi1,2, Kenji Sugiyama2,3, Shoko Saito2, Akiko Numajiri2 and Kyosuke Nagata*,2

University of Marmara, Faculty of Pharmacy, Department of Pharmaceutical Biotechnology, Haydarpasa, Kadikoy, Istanbul 34668, Turkey, 1 Department of Immunology and Microbiology, Nihon University, School of Medicine, 30-1 Oyaguchi-Kamimachi, Itabashi-ku, Tokyo 173-8610, Japan, 2 Department of Infection Biology, Institute of Basic Medical Sciences, University of Tsukuba, 1-1-1 Tennohdai, Tsukuba 305-8575, Japan and 3 Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta, Yokohama 226-8501, Japan

*To whom correspondence should be addressed. Tel: +81 298 53 3233; Fax: +81 298 53 3134; Email: knagata{at}md.tsukuba.ac.jp
The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors

Mx proteins belong to the dynamin superfamily of high molecular weight GTPases and interfere with multiplication of a wide variety of viruses. Earlier studies show that nuclear mouse Mx1 and human MxA designed to be localized in the nucleus inhibit the transcription step of the influenza virus genome. Here we set a transient influenza virus transcription system using luciferase as a reporter gene and cells expressing the three RNA polymerase subunits, PB1, PB2 and PA, and NP. We used this reporter assay system and nuclear-localized MxA proteins to get clues for elucidating the anti-influenza virus activity of MxA. Nuclear-localized VP16-MxA and MxA-TAg NLS strongly interfered with the influenza virus transcription. Over-expression of PB2 led to a slight resumption of the transcription inhibition by nuclear MxA, whereas over-expression of PB1 and PA did not affect the MxA activity. Of interest is that the inhibitory activity of the nuclear MxA was markedly neutralized by over-expression of NP. An NP devoid of its C-terminal region, but containing the N-terminal RNA binding domain, also neutralized the VP16-MxA activity in a dose-dependent manner, whereas an NP lacking the N-terminal region did not affect the VP16-MxA activity. Further, not only VP16-MxA but also the wild-type MxA was found to interact with NP in influenza virus-infected cells. This indicates that the nuclear MxA suppresses the influenza virus transcription by interacting with not only PB2 but also NP.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Immunol.Home page
M. Schmolke, D. Viemann, J. Roth, and S. Ludwig
Essential Impact of NF-{kappa}B Signaling on the H5N1 Influenza A Virus-Induced Transcriptome
J. Immunol., October 15, 2009; 183(8): 5180 - 5189.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
C. T. O. Benfield, J. W. Lyall, G. Kochs, and L. S. Tiley
Asparagine 631 Variants of the Chicken Mx Protein Do Not Inhibit Influenza Virus Replication in Primary Chicken Embryo Fibroblasts or In Vitro Surrogate Assays
J. Virol., August 1, 2008; 82(15): 7533 - 7539.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
J. Dittmann, S. Stertz, D. Grimm, J. Steel, A. Garcia-Sastre, O. Haller, and G. Kochs
Influenza A Virus Strains Differ in Sensitivity to the Antiviral Action of Mx-GTPase
J. Virol., April 1, 2008; 82(7): 3624 - 3631.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. D. Carroll, S. R. Matzinger, M. Genesca, L. Fritts, R. Colon, M. B. McChesney, and C. J. Miller
Interferon-Induced Expression of MxA in the Respiratory Tract of Rhesus Macaques Is Suppressed by Influenza Virus Replication
J. Immunol., February 15, 2008; 180(4): 2385 - 2395.
[Abstract] [Full Text] [PDF]

Home page
 GENES CELLSHome page
A. Honda, T. Okamoto, and A. Ishihama
Host factor Ebp1: Selective inhibitor of influenza virus transcriptase.
Genes Cells, February 1, 2007; 12(2): 133 - 142.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
A. Kawaguchi, T. Naito, and K. Nagata
Involvement of Influenza Virus PA Subunit in Assembly of Functional RNA Polymerase Complexes
J. Virol., January 15, 2005; 79(2): 732 - 744.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.