Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (183K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (7)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by McKinney, J. S.
Right arrow Articles by Altman, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by McKinney, J. S.
Right arrow Articles by Altman, S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Published online 3 February 2004

Nucleic Acids Research, 2004, Vol. 32, No. 2 848-854
© 2004 Oxford University Press

Disruption of type III secretion in Salmonella enterica serovar Typhimurium by external guide sequences

Jeffrey S. McKinney*,1,2, Haifeng Zhang1, Tomoko Kubori3, Jorge E. Galán3 and Sidney Altman1

1 Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA, 2 Department of Pediatrics, Division of Infectious Diseases, Yale University School of Medicine, New Haven, CT 06520, USA and 3 Section of Microbial Pathogenesis, Yale University School of Medicine, New Haven, CT 06536, USA

*To whom correspondence should be addressed. Tel: +1 314 286 2912; Fax: +1 314 286 2895; Email: McKinney_J{at}kids.wustl.edu
Present address:
Jeffrey S. McKinney, Departments of Pediatrics and Molecular Microbiology, Washington University, Saint Louis, MO 63110, USA

The type III secretion system involved in Salmonella enterica serovar Typhimurium invasion of host cells has been disrupted using inducibly expressed oligonucleotide external guide sequences (EGSs) complementary to invB or invC mRNA. These EGSs direct single site cleavage in these mRNAs by endogenous RNase P, and their expression in Salmonella results in invC mRNA and InvC protein depletion, decreased type III secretion and interference with host cell invasion. Comparison of these effects with those from studies of Salmonella invB and invC mutants suggests that invB EGSs have polar effects on invC mRNA.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 RNAHome page
J.-h. Ko, M. Izadjoo, and S. Altman
Inhibition of expression of virulence genes of Yersinia pestis in Escherichia coli by external guide sequences and RNase P
RNA, August 1, 2008; 14(8): 1656 - 1662.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
E. W. Lundblad, G. Xiao, J.-h. Ko, and S. Altman
Rapid selection of accessible and cleavable sites in RNA by Escherichia coli RNase P and random external guide sequences
PNAS, February 19, 2008; 105(7): 2354 - 2357.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
A. Negrea, E. Bjur, S. E. Ygberg, M. Elofsson, H. Wolf-Watz, and M. Rhen
Salicylidene Acylhydrazides That Affect Type III Protein Secretion in Salmonella enterica Serovar Typhimurium
Antimicrob. Agents Chemother., August 1, 2007; 51(8): 2867 - 2876.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
A. J. C. S. Bistue, H. Ha, R. Sarno, M. Don, A. Zorreguieta, and M. E. Tolmasky
External Guide Sequences Targeting the aac(6')-Ib mRNA Induce Inhibition of Amikacin Resistance
Antimicrob. Agents Chemother., June 1, 2007; 51(6): 1918 - 1925.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.