Skip Navigation

Nucleic Acids Research 2004 32(20):6057-6068; doi:10.1093/nar/gkh939
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (853K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (18)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Kim, K.-s.
Right arrow Articles by Lee, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kim, K.-s.
Right arrow Articles by Lee, Y.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Published online 18 November 2004

Nucleic Acids Research, Vol. 32 No. 20 © Oxford University Press 2004; all rights reserved

Regulation of 6S RNA biogenesis by switching utilization of both sigma factors and endoribonucleases

Kwang-sun Kim and Younghoon Lee*

Department of Chemistry and Center for Molecular Design and Synthesis, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea

* To whom correspondence should be addressed. Tel: +82 42 869 2832; Fax: +82 42 869 2810; Email: Younghoon.Lee{at}kaist.ac.kr

Received August 4, 2004; Revised and Accepted October 26, 2004

In Escherichia coli, 6S RNA functions as a modulator of RNA polymerase {sigma}70-holoenzyme activity, but its biosynthetic pathway remains uncharacterized. In this study, to further understand the regulatory circuit of 6S RNA biosynthesis for the modulation of E{sigma}70 activity, we have characterized the biogenesis of 6S RNA. We reveal that there are two different precursors, a long and a short molecule, which are transcribed from the distal P2 and proximal P1 promoter, respectively. Transcription from the P2 promoter is both {sigma}70- and {sigma}S-dependent, whereas, in contrast, P1 transcription is {sigma}70- but not {sigma}S-dependent. Both precursors are processed to generate the 5' end of 6S RNA, and while the long precursor is processed exclusively by RNase E, the short precursor is processed by both RNase G and RNase E. Our data indicate that the switching of the utilization of both sigma factors and endoribonucleases in the biogenesis of 6S RNA would play an essential role in modulating its levels in E.coli.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
D. Singh, S.-J. Chang, P.-H. Lin, O. V. Averina, V. R. Kaberdin, and S. Lin-Chao
Regulation of ribonuclease E activity by the L4 ribosomal protein of Escherichia coli
PNAS, January 20, 2009; 106(3): 864 - 869.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K.-s. Kim, S. Sim, J.-h. Ko, and Y. Lee
Processing of M1 RNA at the 3' End Protects Its Primary Transcript from Degradation
J. Biol. Chem., October 14, 2005; 280(41): 34667 - 34674.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
J. E. BARRICK, N. SUDARSAN, Z. WEINBERG, W. L. RUZZO, and R. R. BREAKER
6S RNA is a widespread regulator of eubacterial RNA polymerase that resembles an open promoter
RNA, May 1, 2005; 11(5): 774 - 784.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
D. K. Willkomm, J. Minnerup, A. Huttenhofer, and R. K. Hartmann
Experimental RNomics in Aquifex aeolicus: identification of small non-coding RNAs and the putative 6S RNA homolog
Nucleic Acids Res., April 6, 2005; 33(6): 1949 - 1960.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.