Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (202K) Freely available
Right arrow Supplementary Material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (15)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Malek, J. A.
Right arrow Articles by McKernan, K. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Malek, J. A.
Right arrow Articles by McKernan, K. J.
Related Collections
Right arrow Protein-protein interaction
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Published online 10 February 2004

Nucleic Acids Research, 2004, Vol. 32, No. 3 1059-1064
© 2004 Oxford University Press

Protein interaction mapping on a functional shotgun sequence of Rickettsia sibirica

Joel A. Malek*, Jamey M. Wierzbowski, Wei Tao, Stephanie A. Bosak, David J. Saranga, Lynn Doucette-Stamm, Douglas R. Smith, Paul J. McEwan and Kevin J. McKernan

Agencourt Bioscience Corporation, 100 Cummings Center Suite 107G, Beverly, MA 01915, USA

*To whom correspondence should be addressed. Tel: +1 978 867 2632; Fax: +1 978 867 2601; Email: jamalek{at}agencourt.com

Protein interaction maps can reveal novel pathways and functional complexes, allowing ‘guilt by association’ annotation of uncharacterized proteins. To address the need for large-scale protein interaction analyses, a bacterial two-hybrid system was coupled with a whole genome shotgun sequencing approach for microbial genome analysis. We report the first large-scale proteomics study using this system, integrating de novo genome sequencing with functional interaction mapping and annotation in a high-throughput format. We apply the approach by shotgun sequencing and annotating the genome of Rickettsia sibirica strain 246, an obligate intracellular human pathogen among the Spotted Fever Group rickettsiae. The bacteria invade endothelial cells and cause lysis after large amounts of progeny have accumulated. Little is known about specific Rickettsial virulence factors and their mode of pathogenicity. Analysis of the combined genomic sequence and protein–protein interaction data for a set of virulence related Type IV secretion system (T4SS) proteins revealed over 250 interactions and will provide insight into the mechanism of Rickettsial pathogenicity.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Bacteriol.Home page
E. Rances, D. Voronin, V. Tran-Van, and P. Mavingui
Genetic and Functional Characterization of the Type IV Secretion System in Wolbachia
J. Bacteriol., July 15, 2008; 190(14): 5020 - 5030.
[Abstract] [Full Text] [PDF]

Home page
 Genome Res.Home page
G. Blanc, H. Ogata, C. Robert, S. Audic, J.-M. Claverie, and D. Raoult
Lateral gene transfer between obligate intracellular bacteria: Evidence from the Rickettsia massiliae genome
Genome Res., November 1, 2007; 17(11): 1657 - 1664.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
R. J. F. Haft, E. G. Gachelet, T. Nguyen, L. Toussaint, D. Chivian, and B. Traxler
In Vivo Oligomerization of the F Conjugative Coupling Protein TraD
J. Bacteriol., September 15, 2007; 189(18): 6626 - 6634.
[Abstract] [Full Text] [PDF]

Home page
 Ann. N. Y. Acad. Sci.Home page
M. E. EREMEEVA, A. MADAN, C. D. SHAW, K. TANG, and G. A. DASCH
New Perspectives on Rickettsial Evolution from New Genome Sequences of Rickettsia, particularly R. canadensis, and Orientia tsutsugamushi
Ann. N.Y. Acad. Sci., December 1, 2005; 1063(1): 47 - 63.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Microbiol. Rev.Home page
P. Parola, C. D. Paddock, and D. Raoult
Tick-Borne Rickettsioses around the World: Emerging Diseases Challenging Old Concepts
Clin. Microbiol. Rev., October 1, 2005; 18(4): 719 - 756.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
P. Clarke, Pár. Cuív, and M. O'Connell
Novel mobilizable prokaryotic two-hybrid system vectors for high-throughput protein interaction mapping in Escherichia coli by bacterial conjugation
Nucleic Acids Res., February 1, 2005; 33(2): e18 - e18.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.