Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (266K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (21)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Richard, D. J.
Right arrow Articles by White, M. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Richard, D. J.
Right arrow Articles by White, M. F.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Published online 10 February 2004

Nucleic Acids Research, 2004, Vol. 32, No. 3 1065-1074
© 2004 Oxford University Press

Physical and functional interaction of the archaeal single-stranded DNA-binding protein SSB with RNA polymerase

Derek J. Richard, Stephen D. Bell1 and Malcolm F. White*

Centre for Biomolecular Sciences, University of St Andrews, North Haugh, St Andrews, Fife KY16 9ST, UK and 1 MRC Cancer Cell Unit, Hutchison MRC Centre, Hills Road, Cambridge CB2 2XZ, UK

*To whom correspondence should be addressed. Tel: +44 1334 463432; Fax: +44 1334 462595; Email: mfw2{at}st-andrews.ac.uk

Archaeal transcription utilizes a complex multisubunit RNA polymerase and the basal transcription factors TBP and TF(II)B, closely resembling its eukaryal counterpart. We have uncovered a tight physical and functional interaction between RNA polymerase and the single-stranded DNA-binding protein SSB in Sulfolobus solfataricus. SSB stimulates transcription from promoters in vitro under TBP-limiting conditions and supports transcription in the absence of TBP. SSB also rescues transcription from repression by reconstituted chromatin. We demonstrate the potential for promoter melting by SSB, suggesting a plausible basis for the stimulation of transcription. This stimulation requires both the single-stranded DNA-binding domain and the acidic C-terminal tail of the SSB. The tail forms a stable interaction with RNA polymerase. These data reveal an unexpected role for single-stranded DNA-binding proteins in transcription in archaea.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
J. Rudolf, C. Rouillon, U. Schwarz-Linek, and M. F. White
The helicase XPD unwinds bubble structures and is not stalled by DNA lesions removed by the nucleotide excision repair pathway
Nucleic Acids Res., November 20, 2009; (2009) gkp1058v1.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. Valenti, G. Perugino, T. Nohmi, M. Rossi, and M. Ciaramella
Inhibition of translesion DNA polymerase by archaeal reverse gyrase
Nucleic Acids Res., July 1, 2009; 37(13): 4287 - 4295.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. Zhang, E. Li, and G. J. Olsen
Protein-coding gene promoters in Methanocaldococcus (Methanococcus) jannaschii
Nucleic Acids Res., June 1, 2009; 37(11): 3588 - 3601.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. M. Baitin, M. C. Gruenig, and M. M. Cox
SSB Antagonizes RecX-RecA Interaction
J. Biol. Chem., May 23, 2008; 283(21): 14198 - 14204.
[Abstract] [Full Text] [PDF]

Home page
 J. Mol. Diagn.Home page
J. Goldmeyer, H. Kong, and W. Tang
Development of a Novel One-Tube Isothermal Reverse Transcription Thermophilic Helicase-Dependent Amplification Platform for Rapid RNA Detection
J. Mol. Diagn., November 1, 2007; 9(5): 639 - 644.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. D. Hobbs, A. Sakai, and M. M. Cox
SSB Protein Limits RecOR Binding onto Single-stranded DNA
J. Biol. Chem., April 13, 2007; 282(15): 11058 - 11067.
[Abstract] [Full Text] [PDF]

Home page
 Microbiol. Mol. Biol. Rev.Home page
E. R. Barry and S. D. Bell
DNA Replication in the Archaea
Microbiol. Mol. Biol. Rev., December 1, 2006; 70(4): 876 - 887.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
I. Mijakovic, D. Petranovic, B. Macek, T. Cepo, M. Mann, J. Davies, P. R. Jensen, and D. Vujaklija
Bacterial single-stranded DNA-binding proteins are phosphorylated on tyrosine
Nucleic Acids Res., March 20, 2006; 34(5): 1588 - 1596.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. Napoli, A. Valenti, V. Salerno, M. Nadal, F. Garnier, M. Rossi, and M. Ciaramella
Functional interaction of reverse gyrase with single-strand binding protein of the archaeon Sulfolobus
Nucleic Acids Res., January 26, 2005; 33(2): 564 - 576.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.