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Published online 10 February 2004

Nucleic Acids Research, 2004, Vol. 32, No. 3 989-996
© 2004 Oxford University Press

The effect of manganese(II) on DNA structure: electronic and vibrational circular dichroism studies

A. M. Polyanichko1,2, V. V. Andrushchenko2, E. V. Chikhirzhina3, V. I. Vorob’ev3 and H. Wieser*,2

1 Department of Physics, St Petersburg State University, St Petersburg, Russian Federation, 2 Department of Chemistry, University of Calgary, Calgary, Canada and 3 Institute of Cytology of the Russian Academy of Sciences, St Petersburg, Russian Federation

*To whom correspondence should be addressed. Tel: +1 403 220 4934; Fax: +1 403 289 7635; Email: hwieser{at}ucalgary.ca

The interaction of DNA with Mn2+ was studied in absorbance and optical activity in the electronic and vibrational regions. Based on the data, several stages of the interaction were identified. Con formational transition towards the C-form of DNA was observed in solution at the molar ratio Mn2+/DNA-phosphates between 0.1 and 1.5. The exact ratio depended on the ionic strength and increased with increasing NaCl concentration. Although manganese interacted with the phosphates and bases of DNA at higher metal concentrations, it is unlikely that direct chelation occurred. A model for the interaction between manganese ions and DNA mediated by water is suggested destabilizing the double helix and partially breaking the hydrogen bonds between the base pairs. At high Mn2+ concentrations DNA aggregation was observed.


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