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Published online 18 February 2004

Nucleic Acids Research, 2004, Vol. 32, No. 3 e33
© 2004 Oxford University Press

Surfection: a new platform for transfected cell arrays

Fu-Hsiung Chang*, Chien-Hsin Lee, Ming-Ta Chen, Chun-Chen Kuo, Yi-Lin Chiang, Chi-Ying Hang1 and Steve Roffler2

Institute of Biochemistry and Molecular Biology, College of Medicine, National Taiwan University, Taipei, Taiwan, 1 Division of Molecular and Genomic Medicine, National Health Research Institutes, Taipei, Taiwan and 2 Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan

*To whom correspondence should be addressed. Tel: +886 2 23216566; Fax: +886 2 23915295; Email: fhchang{at}ha.mc.ntu.edu.tw

Efficient high-throughput expression of genes in mammalian cells can facilitate large-scale functional genomic studies. Towards this aim, we developed a simple yet powerful method to deliver genes into cells by cationic polymers on the surface of substrates. Transfection can be achieved by directly contacting nucleic acid–cell mixtures with the cationic substrates, e.g. polyethylenimine/collagen-coated wells. This single-step matrix-surface- mediated transfection method, termed ‘surfection’, can efficiently deliver multiple plasmids into cells and can successfully assay siRNA-mediated gene silencing. This technology represents the easiest method to transfer combinations of genes in large-scale arrays, and is a versatile tool for live-cell imaging and cell-based drug screening.


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