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Published online 25 February 2004

Nucleic Acids Research, 2004, Vol. 32, No. 4 1363-1371
© 2004 Oxford University Press

ARB: a software environment for sequence data

Wolfgang Ludwig*, Oliver Strunk, Ralf Westram, Lothar Richter, Harald Meier1, Yadhukumar, Arno Buchner, Tina Lai, Susanne Steppi, Gangolf Jobb1, Wolfram Förster1, Igor Brettske, Stefan Gerber, Anton W. Ginhart1, Oliver Gross, Silke Grumann1, Stefan Hermann1, Ralf Jost1, Andreas König1, Thomas Liss1, Ralph Lüßmann1, Michael May1, Björn Nonhoff1, Boris Reichel1, Robert Strehlow1, Alexandros Stamatakis1, Norbert Stuckmann1, Alexander Vilbig1, Michael Lenke1, Thomas Ludwig2, Arndt Bode1 and Karl-Heinz Schleifer

Lehrstuhl für Mikrobiologie, Technische Universität München, D-853530 Freising, Germany 1 Lehrstuhl für Rechnertechnik und Rechnerorganisation, Parallelrechnerarchitektur, Technische Universität München, D-85748 Garching, Germany 2 Institut für Informatik, Ruprecht-Karls-Universität Heidelberg, D-69120 Heidelberg, Germany

*To whom correspondence should be addressed. Tel: +8161 71 5451; Fax: +8161 71 5475; Email: ludwig{at}mikro.biologie.tu-muenchen.de

The ARB (from Latin arbor, tree) project was initiated almost 10 years ago. The ARB program package comprises a variety of directly interacting software tools for sequence database maintenance and analysis which are controlled by a common graphical user interface. Although it was initially designed for ribosomal RNA data, it can be used for any nucleic and amino acid sequence data as well. A central database contains processed (aligned) primary structure data. Any additional descriptive data can be stored in database fields assigned to the individual sequences or linked via local or worldwide networks. A phylogenetic tree visualized in the main window can be used for data access and visualization. The package comprises additional tools for data import and export, sequence alignment, primary and secondary structure editing, profile and filter calculation, phylogenetic analyses, specific hybridization probe design and evaluation and other components for data analysis. Currently, the package is used by numerous working groups worldwide.


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