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Published online 12 March 2004

Nucleic Acids Research, 2004, Vol. 32, No. 5 1688-1695
© 2004 Oxford University Press

Identification of 20 microRNAs from Oryza sativa

Jia-Fu Wang, Hui Zhou, Yue-Qin Chen, Qing-Jun Luo and Liang-Hu Qu*

Key Laboratory of Gene Engineering of the Ministry of Education, Biotechnology Research Center, Zhongshan University, Guangzhou 510275, People’s Republic of China

*To whom correspondence should be addressed. Tel: +86 20 84112399; Fax: +1 86 20 84036551; Email: lsbrc04{at}zsu.edu.cn
The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors

MicroRNAs (miRNAs) are present in both plant and animal kingdoms and represents a growing family of non-coding RNAs. These tiny RNAs act as small guides and direct negative regulations usually in the process of development through sequence complementarity to target mRNAs. Although a large number of miRNAs have been identified from various animals, so far plant miRNA studies have focused mainly on Arabidopsis. Here we describe the identification of 20 miRNAs from a rice cDNA library. All the miRNAs were presumably processed from precursors with stem–loop structures and were positively detected in rice cells from at least one tissue, some of which showed tissue-specific expression. Twenty-three unique rice genes were identified to be feasible targets for seven rice miRNAs, including four members of Scarecrow-like transcription factor, the targets of miR-39 that had been characterized in Arabidopsis. Lacking long complementarity, the regulatory targets of 13 miRNAs remain to be further investigated. A possible mechanism of translational repressor for plant miRNAs that lack perfect complementarity to target mRNAs is discussed.


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