Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (326K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (22)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Meulewaeter, F.
Right arrow Articles by van Eldik, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Meulewaeter, F.
Right arrow Articles by van Eldik, G.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Published online 12 March 2004

Nucleic Acids Research, 2004, Vol. 32, No. 5 1721-1730
© 2004 Oxford University Press

Conservation of RNA structures enables TNV and BYDV 5' and 3' elements to cooperate synergistically in cap-independent translation

Frank Meulewaeter*, Rosalinde van Lipzig, Alexander P. Gultyaev1,2, Cornelis W. A. Pleij1, Daniel Van Damme, Marc Cornelissen and Gerben van Eldik

Bayer BioScience N.V., J. Plateaustraat 22, B-9000, Gent, Belgium, 1 Leiden Institute of Chemistry, Gorlaeus Laboratories, 2300 RA Leiden, The Netherlands and 2 Section Theoretical Biology, Institute of Biology, Leiden University, Kaiserstraat 63, 2311 GP Leiden, The Netherlands

*To whom correspondence should be addressed. Tel: +32 9 235 84 38, Fax: +32 9 224 06 94; Email: frank.meulewaeter{at}bayercropscience.com
Present address:
Daniel Van Damme, Department of Plant Systems Biology, VIB / Ghent University, Technologiepark 927, B-9052 Gent, Belgium

The subgenomic RNA 2 of tobacco necrosis virus A (TNV sgRNA2) encodes the viral coat protein, is unpolyadenylated and presumably uncapped. Here, we show that TNV sgRNA2 is translated cap independently. This cap-independent translation requires the leader and a 140 nt element of the trailer both in wheat germ extract and in tobacco protoplasts. Similar to barley yellow dwarf virus (BYDV), the TNV 5' and 3' elements stimulate translation synergistically. Computer-aided phylogenetic analysis of the secondary structure of the TNV trailer revealed that the 3' translation element is part of a major conserved stem–loop that contains similarities to structures in the BYDV 3' translation element. These data suggest that the translation mechanisms of TNV sgRNA2 and BYDV RNA are related. To further characterize this relationship, we tested whether cooperativity exists between TNV sgRNA2 and BYDV 5' and 3' elements. We found that the TNV sgRNA2 5' element stimulates translation synergistically with the BYDV 3' element in vitro. This finding is the first evidence for conservation of structures that enable a 5'–3' interaction stimulating cap-independent translation.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Gen. Virol.Home page
X. Yuan, Y. Cao, D. Xi, L. Guo, C. Han, D. Li, Y. Zhai, and J. Yu
Analysis of the subgenomic RNAs and the small open reading frames of Beet black scorch virus.
J. Gen. Virol., October 1, 2006; 87(Pt 10): 3077 - 3086.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
A. M. Rakotondrafara, C. Polacek, E. Harris, and W. A. Miller
Oscillating kissing stem-loop interactions mediate 5' scanning-dependent translation by a viral 3'-cap-independent translation element
RNA, October 1, 2006; 12(10): 1893 - 1906.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
D. Edgil, C. Polacek, and E. Harris
Dengue Virus Utilizes a Novel Strategy for Translation Initiation When Cap-Dependent Translation Is Inhibited
J. Virol., March 15, 2006; 80(6): 2976 - 2986.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
Y.-H. Hsu, H.-C. Chen, J. Cheng, P. Annamali, B.-Y. Lin, C.-T. Wu, W.-B. Yeh, and N.-S. Lin
Crucial Role of the 5' Conserved Structure of Bamboo Mosaic Virus Satellite RNA in Downregulation of Helper Viral RNA Replication
J. Virol., March 1, 2006; 80(5): 2566 - 2574.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. R. Fabian and K. A. White
5'-3' RNA-RNA Interaction Facilitates Cap- and Poly(A) Tail-independent Translation of Tomato Bushy Stunt Virus mRNA: A POTENTIAL COMMON MECHANISM FOR TOMBUSVIRIDAE
J. Biol. Chem., July 9, 2004; 279(28): 28862 - 28872.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.