Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (232K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (8)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Glossop, J. A.
Right arrow Articles by Roberts, S. G. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Glossop, J. A.
Right arrow Articles by Roberts, S. G. E.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Published online 22 March 2004

Nucleic Acids Research, 2004, Vol. 32, No. 5 1829-1835
Oxford University Press

A conformational change in TFIIB is required for activator-mediated assembly of the preinitiation complex

James A. Glossop, Tim R. Dafforn and Stefan G. E. Roberts*

School of Biological Sciences, The Michael Smith Building, University of Manchester, Oxford Road, Manchester M13 9PT, UK

*To whom correspondence should be addressed. Tel: +44 161 275 5758; Fax: +44 161 275 5600; Email: stefan.roberts{at}man.ac.uk

Received January 26, 2004; Revised and Accepted March 4, 2004

TFIIB plays a pivotal role during assembly of the RNA polymerase II transcription preinitiation complex. TFIIB is composed of two domains that engage in an intramolecular interaction that can be disrupted by the VP16 activation domain. In this study, we describe a novel human TFIIB derivative harbouring two point mutations in the highly conserved N-terminal charged cluster domain. This mutant, TFIIB R53E:R66E, exhibits an enhanced affinity in its intramolecular interaction when compared with wild-type TFIIB. Consistent with this, the mutant displays a significantly reduced affinity for VP16. However, its ability to complex with TATA-binding protein at a model promoter is equivalent to that of wild-type TFIIB. Furthermore, this TFIIB derivative is able to support high order preinitiation complex assembly in the absence of an activator. Strikingly though, an activator fails to recruit the TFIIB mutant to the promoter. Taken together, our results show that a TFIIB conformational change is critical for the formation of activator-dependent transcription complexes.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
W. Deng, B. Malecova, T. Oelgeschlager, and S. G. E. Roberts
TFIIB Recognition Elements Control the TFIIA-NC2 Axis in Transcriptional Regulation
Mol. Cell. Biol., March 15, 2009; 29(6): 1389 - 1400.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
L. M. Green, K. J. Wagner, H. A. Campbell, K. Addison, and S. G. E. Roberts
Dynamic interaction between WT1 and BASP1 in transcriptional regulation during differentiation
Nucleic Acids Res., February 1, 2009; 37(2): 431 - 440.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J. Seyfried, X. Wang, G. Kharebava, and C. Tournier
A Novel Mitogen-Activated Protein Kinase Docking Site in the N Terminus of MEK5{alpha} Organizes the Components of the Extracellular Signal-Regulated Kinase 5 Signaling Pathway
Mol. Cell. Biol., November 15, 2005; 25(22): 9820 - 9828.
[Abstract] [Full Text] [PDF]

Home page
 GENES CELLSHome page
K. Hayashi, T. Watanabe, A. Tanaka, T. Furumoto, C. Sato-Tsuchiya, M. Kimura, M. Yokoi, A. Ishihama, F. Hanaoka, and Y. Ohkuma
Studies of Schizosaccharomyces pombe TFIIE indicate conformational and functional changes in RNA polymerase II at transcription initiation
Genes Cells, March 1, 2005; 10(3): 207 - 224.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
R. T. Hori, S. Xu, X. Hu, and S. Pyo
TFIIB-facilitated recruitment of preinitiation complexes by a TAF-independent mechanism
Nucleic Acids Res., July 22, 2004; 32(13): 3856 - 3863.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.