Published online 15 April 2004
Nucleic Acids Research, 2004, Vol. 32, No. 7 2069-2078
© 2004 Oxford University Press
Differential evolution of the Saccharomyces cerevisiae DUP240 paralogs and implication of recombination in phylogeny
Laboratoire de Microbiologie et Génétique, FRE 2326 Université Louis Pasteur/CNRS, Institut de Botanique, F-67083 Strasbourg Cedex, France and 1 Unité de Rétrovirologie Moléculaire, Institut Pasteur, F-75724 Paris Cedex 15, France
*To whom correspondence should be addressed. Tel: +33 3 90 24 18 17; Fax: +33 3 90 24 20 28; Email: souciet{at}gem.u-strasbg.fr
The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors
Received November 18, 2003; Revised February 5, 2004; Accepted March 16, 2004
Multigene families are observed in all genomes sequenced so far and are the reflection of key evolutionary mechanisms. The DUP240 family, identified in Saccharomyces cerevisiae strain S288C, is composed of 10 paralogs: seven are organized as two tandem repeats and three are solo ORFs. To investigate the evolution of the three solo paralogs, YAR023c, YCR007c and YHL044w, we performed a comparative analysis between 15 S.cerevisiae strains. These three ORFs are present in all strains and the conservation of synteny indicates that they are not frequently involved in chromosomal reshaping, in contrast to the DUP240 ORFs organized in tandem repeats. Our analysis of nucleotide and amino acid variations indicates that YAR023c and YHL044w fix mutations more easily than YCR007c, although they all belong to the same multigene family. This comparative analysis was also conducted with five arbitrarily chosen Ascomycetes-specific genes and five arbitrarily chosen common genes (genes that have a homolog in at least one non-Ascomycetes organism). Ascomycetes-specific genes appear to be diverging faster than common genes in the S.cerevisiae species, a situation that was previously described between different yeast species. Our results point to the strong contribution, during DNA sequence evolution, of allelic recombination besides nucleotide substitution.
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