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Published online 23 April 2004

Nucleic Acids Research, 2004, Vol. 32, No. 7 e68
© 2004 Oxford University Press

Attachment of oligonucleotide probes to poly carbodiimide-coated glass for microarray applications

Naoki Kimura*, Ryuichi Oda, Yoshiaki Inaki1 and Osamu Suzuki

Research and Development Center, Nisshinbo Industries Inc., 1-2-3 Ohnodai, Midori-ku, Chiba-shi, Chiba 267-0056, Japan and 1 Material and Life Science, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan

*To whom correspondence should be addressed. Tel: +81 43 205 0794; Fax: +81 43 205 0843; Email: n-kimu{at}nisshinbo.co.jp

Received October 28, 2003; Revised and Accepted March 19, 2004

Oligonucleotide-based DNA microarrays are becoming increasingly useful tools for the analysis of gene expression and single nucleotide polymorphisms (SNPs). Here, we present a method that permits the manufacture of microarrays from non-modified oligonucleotides on a poly carbodiimide-coated glass surface by UV-irradiation. The use of UV-irradiation facilitates an increase in the level of signal intensity, but it does not affect signal discrimination by the oligonucleotides immobilized on the surface. The signal intensity obtained for an array fabricated using non-modified oligonucleotides with UV-irradiation is ~7-fold greater than that without UV-irradiation. The detection of SNPs was tested to ascertain whether this technique could discriminate specific hybridization signals without causing significant UV-irradiation-induced damage to the immobilized oligonucleotides. We found that this immobilization method provides greater hybridization signals and a better match/mismatch ratio of SNPs than do the established aminosilane techniques. Application of this technology to manufacturing DNA microarrays for sequence analysis is discussed.


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