Published online 26 April 2004
Nucleic Acids Research, 2004, Vol. 32, No. 8 2298-2305
© 2004 Oxford University Press
1-induced DNA bending is required for transcriptional activation by the Mcm1–1 complex
Waksman Institute and Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, NJ 08854, USA
*To whom correspondence should be addressed. Tel: +1 732 445 2905; Fax: +1 732 445 5735; Email: vershon{at}waksman.rutgers.edu
Received December 23, 2003; Revised and Accepted April 1, 2004
The yeast Mcm1 protein is a founding member of the MADS-box family of transcription factors that is involved in the regulation of diverse sets of genes through interactions with distinct cofactor proteins. Mcm1 interacts with the Mat
1 protein to activate the expression of the -cell type-specific genes. To understand the requirement of the cofactor 1 for Mcm1–1-dependent transcriptional activation we analyzed the recruitment of Mcm1 to the promoters of -specific genes in vivo and found that Mcm1 is able to bind to the promoters of -specific genes in the absence of 1. This suggests the function of 1 is more complex than simply recruiting Mcm1. Several MADS-box transcription factors, including Mcm1, induce DNA bending and there is evidence the proper bend may be required for transcriptional activation. We analyzed Mcm1-dependent bending of a Mcm1–1 binding site in the presence and absence of 1 and found that Mcm1 alone shows a reduced DNA-bend at this site compared with other Mcm1 binding sites. However, the addition of 1 markedly increases the DNA-bend and we present evidence this bend is required for full transcriptional activation. These results support a model in which proper DNA-bending by the Mcm1–1 complex is required for transcriptional activation.
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