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Published online 20 May 2004

Nucleic Acids Research, 2004, Vol. 32, No. 9 2873-2879
© 2004 Oxford University Press

Microfluidic device reads up to four consecutive base pairs in DNA sequencing-by-synthesis

Emil P. Kartalov and Stephen R. Quake*

Applied Physics Department, California Institute of Technology, Pasadena, CA 91125, USA

*To whom correspondence should be addressed. Tel: +1 626 395 3362; Fax: +1 626 793 8675; Email: quake{at}caltech.edu

Received February 27, 2004; Revised and Accepted April 26, 2004

We have developed the first fully integrated microfluidic system for DNA sequencing-by-synthesis. Using this chip and fluorescence detection, we have reliably sequenced up to 4 consecutive bps. The described sequencer can be integrated with other microfluidic components on the same chip to produce true lab-on-a-chip technology. The surface chemistry that was designed to anchor the DNA to elastomeric microchannels is useful in a broad range of studies and applications.


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