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Published online 21 May 2004

Nucleic Acids Research, 2004, Vol. 32, No. 9 e77
© 2004 Oxford University Press

Quantitative analysis of highly parallel transfection in cell microarrays

Sandrine Baghdoyan, Yoann Roupioz, Amandine Pitaval, David Castel, Elena Khomyakova1, Alexandre Papine1, Françoise Soussaline1 and Xavier Gidrol*

Service de Génomique Fonctionnelle CEA, 2 Rue Gaston Crémieux CP22, 91057 Evry Cedex, France and 1 IMSTAR, 60 Rue Notre Dame des Champs, 75006 Paris, France

*To whom correspondence should be addressed. Tel: +33 1 60 87 34 74; Fax: +33 1 60 87 34 98; Email: xavier.gidrol{at}cea.fr
The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors

Received January 14, 2004; Revised April 5, 2004;; Accepted April 29, 2004

As more genomes are sequenced, we are facing the challenge of rapidly unraveling the functions of genes. To that end, cell microarrays have recently been described that transfect thousands of nucleic acids in parallel and can be used to analyze the phenotypic consequences of such perturbations. As many parameters can influence the efficacy of transfection in such a format, we describe some important features in manufacturing cell microarrays that may improve reliability and efficiency of both plasmid DNA and siRNA transfection. We have also developed image analysis software that allows automatic detection of cell clusters, quantification of transfection efficiency and levels of expression/extinction of genes. Along with cell microarrays, this bioinformatic tool should expedite functional exploration of the human genome.


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