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Nucleic Acids Research, 2004, Vol. 32, Database issue D418-D420
© 2004 Oxford University Press

Flytrap, a database documenting a GFP protein-trap insertion screen in Drosophila melanogaster

Reed J. Kelso, Michael Buszczak1, Ana T. Quiñones2, Claudia Castiblanco2, Stacy Mazzalupo2 and Lynn Cooley*,2,3

Department of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, University of California, San Francisco, CA 94143, USA, 1 Department of Embryology, Carnegie Institution of Washington, Baltimore, MD 21210, USA, 2 Department of Genetics and 3 Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520-8005, USA

*To whom correspondence should be addressed. Tel: +1 203 785 5067; Fax: +1 203 785 6333; Email: lynn.cooley{at}yale.edu

Flytrap is a web-enabled relational database of transposable element insertions in Drosophila melanogaster. A green fluorescent protein (GFP) artificial exon carried by a transposable P-element is mobilized and inserted into a host gene intron creating a GFP fusion protein. The sequence of the tagged gene is determined by sequencing inverse-PCR products derived from genomic DNA. Flytrap contains two principle data types: micrographs of protein localization and a cellular component ontology, based on rules derived from the Gene Ontology consortium (http://www.geneontology.org), describing protein localization. Flytrap also has links to gene information contained in Flybase (http:// flybase.bio.indiana.edu). The system is designed to accept submissions of micrographs and descriptions from any type of tissue (e.g. wing imaginal disk, ovary) and at any stage of development. Insertion lines can be searched using a number of queries, including Berkeley Drosophila Genome Project (BDGP) numbers and protein localization. In addition, Flytrap provides online order forms linked to each insertion line so that users may request any line generated from this project. Flytrap may be accessed from the homepage at http://flytrap.med. yale.edu.


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