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Nucleic Acids Research 2004 32(Web Server Issue):W121-W123; doi:10.1093/nar/gkh360
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© 2004, the authors
Nucleic Acids Research, Vol. 32, Web Server issue © Oxford University Press 2004; all rights reserved

TROD: T7 RNAi Oligo Designer

Peter Dudek and Didier Picard*

Département de Biologie Cellulaire, Université de Genève, Sciences III, Quai Ernest-Ansermet 30, CH-1211 Genève 4, Switzerland

* To whom correspondence should be addressed. Tel: +41 22 379 6813; Fax: +41 22 379 6928; Email: picard{at}cellbio.unige.ch

Received January 20, 2004; Revised and Accepted March 1, 2004

We have developed T7 RNAi Oligo Designer (TROD), a web application for RNA interference studies. TROD greatly facilitates the design of oligodeoxynucleotide sequences for the in vitro production of siRNA duplexes with T7 RNA polymerase. Given a query cDNA sequence, the program scans for appropriate target sequences based on the constraints of the T7 RNA polymerase method and published criteria for RNA interference with siRNAs. The output is an ordered and prioritized list of ready-to-order DNA oligonucleotide sequences, with links to perform a BLAST search to ascertain target sequence specificity. The TROD web service is available at http://www.cellbio.unige.ch/RNAi.html.

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