Skip Navigation

Nucleic Acids Research 2004 32(Web Server Issue):W121-W123; doi:10.1093/nar/gkh360
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (181K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Dudek, P.
Right arrow Articles by Picard, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dudek, P.
Right arrow Articles by Picard, D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2004, the authors
Nucleic Acids Research, Vol. 32, Web Server issue © Oxford University Press 2004; all rights reserved

TROD: T7 RNAi Oligo Designer

Peter Dudek and Didier Picard*

Département de Biologie Cellulaire, Université de Genève, Sciences III, Quai Ernest-Ansermet 30, CH-1211 Genève 4, Switzerland

* To whom correspondence should be addressed. Tel: +41 22 379 6813; Fax: +41 22 379 6928; Email: picard{at}cellbio.unige.ch

Received January 20, 2004; Revised and Accepted March 1, 2004

We have developed T7 RNAi Oligo Designer (TROD), a web application for RNA interference studies. TROD greatly facilitates the design of oligodeoxynucleotide sequences for the in vitro production of siRNA duplexes with T7 RNA polymerase. Given a query cDNA sequence, the program scans for appropriate target sequences based on the constraints of the T7 RNA polymerase method and published criteria for RNA interference with siRNAs. The output is an ordered and prioritized list of ready-to-order DNA oligonucleotide sequences, with links to perform a BLAST search to ascertain target sequence specificity. The TROD web service is available at http://www.cellbio.unige.ch/RNAi.html.

The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Brief BioinformHome page
J. E. Stajich and H. Lapp
Open source tools and toolkits for bioinformatics: significance, and where are we?
Brief Bioinform, September 1, 2006; 7(3): 287 - 296.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
E. W. Klee, K. J. Shim, M. A. Pickart, S. C. Ekker, and L. B. M. Ellis
AMOD: a morpholino oligonucleotide selection tool
Nucleic Acids Res., July 1, 2005; 33(suppl_2): W506 - W511.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.