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Nucleic Acids Research 2004 32(Web Server Issue):W170-W172; doi:10.1093/nar/gkh369
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© 2004, the authors
Nucleic Acids Research, Vol. 32, Web Server issue © Oxford University Press 2004; all rights reserved

SNPbox: web-based high-throughput primer design from gene to genome

Stefan Weckx, Peter De Rijk, Christine Van Broeckhoven and Jurgen Del-Favero*

Department of Molecular Genetics, Bioinformatics Unit, Flanders Interuniversity Institute for Biotechnology, University of Antwerp, Belgium

* To whom correspondence should be addressed. Tel: +32 3 820 2321; Fax: +32 3 820 2541; Email: jurgen.delfavero{at}ua.ac.be

Received February 12, 2004; Revised and Accepted March 9, 2004

SNPbox is a modular software package that automates the design of PCR primers for large-scale amplification and sequencing projects in a standardized manner resulting in high-quality PCR amplicons with a low failure rate. Here, we present the SNPbox web server at http://www.SNPbox.org, which hosts the SNPbox web service as well as the data from SNPbox analysis of all Ensembl exons. The data of this genome-wide SNPbox application can be visualized in Ensembl's ContigView through a DAS (distributed annotation system) annotation server.

The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated.


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