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Nucleic Acids Research 2005 33(1):e8; doi:10.1093/nar/gni009
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Published online 13 January 2005

© 2005, the authors Nucleic Acids Research, Vol. 33 No. 1 © Oxford University Press 2005; all rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use permissions, please contact journals.permissions{at}oupjournals.org.

Methods Online

Use of a restriction enzyme-digested PCR product as substrate for helicase assays

Jae-Ho Shin, John N. Reeve1 and Zvi Kelman*

University of Maryland Biotechnology Institute, Center for Advanced Research in Biotechnology 9600 Gudelsky Drive, Rockville, MD 20850, USA 1 Department of Microbiology, The Ohio State University Columbus, OH 43210, USA

*To whom correspondence should be addressed. Tel: +1 301 738 6294; Fax: +1 301 738 6255; Email: kelman{at}umbi.umd.edu

Received November 1, 2004. Revised December 1, 2004. Accepted December 14, 2004.

DNA helicases play essential roles in many cellular processes. The currently available techniques to generate substrates for helicase assays are fairly complicated and need some expertise not available in all laboratories. Here, a PCR-based method to generate a substrate for a helicase assay is described, and its application for several archaeal, bacterial and viral enzymes is demonstrated.


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