Skip Navigation

Nucleic Acids Research 2005 33(12):3763-3771; doi:10.1093/nar/gki680
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (1082K) Freely available
Right arrow Screen PDF (332K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (11)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Liu, Z.
Right arrow Articles by Zhang, J.-T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Liu, Z.
Right arrow Articles by Zhang, J.-T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Published online 8 July 2005

© The Author 2005. Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions{at}oupjournals.org

Article

Regulation of expression by promoters versus internal ribosome entry site in the 5'-untranslated sequence of the human cyclin-dependent kinase inhibitor p27kip1

Zhaoqian Liu, Zizheng Dong, Baoguang Han, Youyun Yang, Yang Liu and Jian-Ting Zhang*

Department of Pharmacology and Toxicology, Indiana University Cancer Center, Walther Oncology Center/Walther Cancer Institute, Indiana University School of Medicine 1044 W. Walnut Street, Indianapolis, IN 46202, USA

*To whom correspondence should be addressed. Tel: +1 317 278 4503; Fax: +1 317 274 8046; Email: jianzhan{at}iupui.edu

Received May 15, 2005. Accepted June 12, 2005.

p27kip1 regulates cell proliferation by binding to and inhibiting the activity of cyclin-dependent kinases and its expression oscillates with cell cycle. Recently, it has been suggested from studies using the traditional dicistronic DNA assay that the expression of p27kip1 is regulated by internal ribosome entry site (IRES)-mediated translation initiation, and several RNA-binding protein factors were thought to play some role in this regulation. Considering the inevitable drawbacks of the dicistronic DNA assay, which could mislead a promoter activity or alternative splicing to IRES as previously demonstrated, we decided to reanalyze the 5'-untranslated region (5'-UTR) sequence of p27kip1 and test whether it contains an IRES element or a promoter using more stringent methods, such as dicistronic RNA and promoterless dicistronic and monocistronic DNA assays. We found that the 5'-UTR sequence of human p27kip1 does not have any significant IRES activity. The previously observed IRES activities are likely generated from the promoter activities present in the 5'-UTR sequences of p27kip1. The findings in this study indicate that transcriptional regulation likely plays an important role in p27kip1 expression, and the mechanism of regulation of p27 expression by RNA-binding factors needs to be re-examined. The findings in this study also further enforce the importance that more stringent studies, such as promoterless dicistronic and monocistronic DNA and dicistronic RNA tests, are required to safeguard any future claims of cellular IRES.

Present address: Zhaoqian Liu, Institute of Clinical Pharmacology, Central South University Xiangya School of Medicine, Changsha, People's Republic of China 410078

The authors wish to be known that, in their opinion, the first two authors should be regarded as joint First Authors


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
R. Cuesta, A. Martinez-Sanchez, and F. Gebauer
miR-181a Regulates Cap-Dependent Translation of p27kip1 mRNA in Myeloid Cells
Mol. Cell. Biol., May 15, 2009; 29(10): 2841 - 2851.
[Abstract] [Full Text] [PDF]

Home page
 haematolHome page
G. Desplanques, N. Giuliani, R. Delsignore, V. Rizzoli, R. Bataille, and S. Barille-Nion
Impact of XIAP protein levels on the survival of myeloma cells
Haematologica, January 1, 2009; 94(1): 87 - 93.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
S. Audigier, J. Guiramand, L. Prado-Lourenco, C. Conte, I. G. Gonzalez-Herrera, C. Cohen-Solal, M. Recasens, and A.-C. Prats
Potent activation of FGF-2 IRES-dependent mechanism of translation during brain development
RNA, September 1, 2008; 14(9): 1852 - 1864.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
D. Wei, M. Kanai, Z. Jia, X. Le, and K. Xie
Kruppel-like Factor 4 Induces p27Kip1 Expression in and Suppresses the Growth and Metastasis of Human Pancreatic Cancer Cells
Cancer Res., June 15, 2008; 68(12): 4631 - 4639.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
T. Araud, R. Genolet, P. Jaquier-Gubler, and J. Curran
Alternatively spliced isoforms of the human elk-1 mRNA within the 5' UTR: implications for ELK-1 expression
Nucleic Acids Res., July 9, 2007; 35(14): 4649 - 4663.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
H. Jiang, J. Coleman, R. Miskimins, R. Srinivasan, and W. K. Miskimins
Cap-independent translation through the p27 5'-UTR
Nucleic Acids Res., July 9, 2007; 35(14): 4767 - 4778.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
S. D. Baird, M. Turcotte, R. G. Korneluk, and M. Holcik
Searching for IRES
RNA, October 1, 2006; 12(10): 1755 - 1785.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
A. G. Bert, R. Grepin, M. A. Vadas, and G. J. Goodall
Assessing IRES activity in the HIF-1{alpha} and other cellular 5' UTRs
RNA, June 1, 2006; 12(6): 1074 - 1083.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. Mokrejs, V. Vopalensky, O. Kolenaty, T. Masek, Z. Feketova, P. Sekyrova, B. Skaloudova, V. Kriz, and M. Pospisek
IRESite: the database of experimentally verified IRES structures (www.iresite.org)
Nucleic Acids Res., January 1, 2006; 34(suppl_1): D125 - D130.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. Kozak
A second look at cellular mRNA sequences said to function as internal ribosome entry sites
Nucleic Acids Res., November 28, 2005; 33(20): 6593 - 6602.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.