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Nucleic Acids Research 2005 33(12):e105; doi:10.1093/nar/gni106
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Published online 7 July 2005

© The Author 2005. Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions{at}oupjournals.org

Methods Online

A universal BMV-based RNA recombination system—how to search for general rules in RNA recombination

Magdalena Alejska, Magdalena Figlerowicz1, Nelli Malinowska, Anna Urbanowicz and Marek Figlerowicz*

Institute of Bioorganic Chemistry Polish Academy of Sciences Noskowskiego 12/14, 61-704 Poznan, Poland 1Department of Infectious Diseases and Child Neurology, University of Medical Sciences Szpitalna 27/33, 60-572 Poznan, Poland

*To whom correspondence should be addressed. Tel: +48 61 8528503; Fax: +48 61 8520532; Email: marekf{at}ibch.poznan.pl

Received February 14, 2005. Revised May 11, 2005. Accepted June 16, 2005.

At present, there is no doubt that RNA recombination is one of the major factors responsible for the generation of new RNA viruses and retroviruses. Numerous experimental systems have been created to investigate this complex phenomenon. Consequently, specific RNA structural motifs mediating recombination have been identified in several viruses. Unfortunately, up till now a unified model of genetic RNA recombination has not been formulated, mainly due to difficulties with the direct comparison of data obtained for different RNA-based viruses. To solve this problem, we have attempted to construct a universal system in which the recombination activity of various RNA sequences could be tested. To this end, we have used brome mosaic virus, a model (+)RNA virus of plants, for which the structural requirements of RNA recombination are well defined. The effectiveness of the new homomolecular system has been proven in an experiment involving two RNA sequences derived from the hepatitis C virus genome. In addition, comparison of the data obtained with the homomolecular system with those generated earlier using the heteromolecular one has provided new evidence that the mechanisms of homologous and non-homologous recombination are different and depend on the virus' mode of replication.


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