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Nucleic Acids Research 2005 33(13):4157-4163; doi:10.1093/nar/gki733
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Published online 26 July 2005

© The Author 2005. Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions{at}oupjournals.org

Article

Induction of transcription within chromosomal DNA loops flanked by MAR elements causes an association of loop DNA with the nuclear matrix

Olga V. Iarovaia, Sergey B. Akopov1, Lev G. Nikolaev1, Eugene D. Sverdlov1 and Sergey V. Razin*

Laboratory of Structural and Functional Organization of Chromosomes, Institute of Gene Biology RAS Vavilov Street 34/5, 117984 Moscow, Russia 1Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS 117997 Moscow, Russia

*To whom correspondence should be addressed. Tel: +7 95 135 30 92; Fax: +7 95 135 41 05; Email: sergey.v.razin{at}usa.net

Received May 19, 2005. Revised June 23, 2005. Accepted July 8, 2005.

The spatial organization of an ~170 kb region of human chromosome 19, including CD22 and GPR40–GPR43 genes, was studied using in situ hybridization of a set of cosmid and PAC probes with nuclear halos prepared from proliferating and differentiated HL60 cells. The whole region under study was found to be looped out into the nuclear halo in proliferating cells. It is likely that the loop observed was attached to the nuclear matrix via MAR elements present at the flanks of the area under study. Upon dimethyl sulfoxide-induced differentiation of the cells the looped fragment became associated with the nuclear matrix. This change in the spatial organization correlated with the activation of transcription of at least two (CD22 and GPR43) genes present within the loop. The data obtained are discussed in the framework of the hypothesis postulating that the spatial organization of chromosomal DNA is maintained via constitutive (basic) and facultative (transcription-related) interactions of the latter with the nuclear matrix.


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