Skip Navigation

Nucleic Acids Research 2005 33(14):4379-4394; doi:10.1093/nar/gki751
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (3080K) Freely available
Right arrow Screen PDF (787K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (23)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Pines, A.
Right arrow Articles by Tell, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pines, A.
Right arrow Articles by Tell, G.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Published online 2 August 2005

© The Author 2005. Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions{at}oupjournals.org

Article

Activation of APE1/Ref-1 is dependent on reactive oxygen species generated after purinergic receptor stimulation by ATP

Alex Pines, Lorena Perrone1, Nicoletta Bivi, Milena Romanello, Giuseppe Damante, Massimo Gulisano1, Mark R. Kelley2, Franco Quadrifoglio and Gianluca Tell*

Department of Biomedical Sciences and Technologies, University of Udine 33100 Udine, Italy 1Department of Physiological Sciences, University of Catania, 95100 Catania, Italy 2Department of Pediatrics Herman B Wells Center for Pediatric Research 1044 W. Walnut Bldg., Indianapolis, IN, USA

*To whom correspondence should be addressed. Tel: +39 0432 494311; Fax: +39 0432 494301; Email: gtell{at}mail.dstb.uniud.it

Received April 26, 2005. Revised July 18, 2005. Accepted July 18, 2005.

Apurinic apyrimidinic endonuclease redox effector factor-1 (APE1/Ref-1) is involved both in the base excision repair (BER) of DNA lesions and in the eukaryotic transcriptional regulation. APE1/Ref-1 is regulated at both the transcriptional and post-translational levels, through control of subcellular localization and post-translational modification. In response to stress conditions, several cell types release ATP, which exerts stimulatory effects on eukaryotic cells via the purinergic receptors (P2) family. By using western blot and immunofluorescence analysis on a human tumour thyroid cell line (ARO), we demonstrate that purinergic stimulation by extracellular ATP induces quick cytoplasm to nucleus translocation of the protein at early times and its neosynthesis at later times. Continuous purinergic triggering by extracellular ATP released by ARO cells is responsible for the control of APE1/Ref-1 intracellular level. Interference with intracellular pathways activated by P2 triggering demonstrates that Ca2+ mobilization and intracellular reactive oxygen species (ROS) production are responsible for APE1/Ref-1 translocation. The APE1/Ref-1 activities on activator protein-1 (AP-1) DNA binding and DNA repair perfectly match its nuclear enrichment upon ATP stimulation. The biological relevance of our data is reinforced by the observation that APE1/Ref-1 stimulation by ATP protects ARO cells by H2O2-induced cell death. Our data provide new insights into the complex mechanisms regulating APE1/Ref-1 functions.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
F. Grassi, G. Tell, M. Robbie-Ryan, Y. Gao, M. Terauchi, X. Yang, M. Romanello, D. P. Jones, M. N. Weitzmann, and R. Pacifici
Oxidative stress causes bone loss in estrogen-deficient mice through enhanced bone marrow dendritic cell activation
PNAS, September 18, 2007; 104(38): 15087 - 15092.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
S. El-Mouatassim, S. Bilotto, G. L. Russo, E. Tosti, and Y. Menezo
APEX/Ref-1 (apurinic/apyrimidic endonuclease DNA-repair gene) expression in human and ascidian (Ciona intestinalis) gametes and embryos
Mol. Hum. Reprod., August 1, 2007; 13(8): 549 - 556.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
G.-M. Zou, M.-H. Luo, A. Reed, M. R. Kelley, and M. C. Yoder
Ape1 regulates hematopoietic differentiation of embryonic stem cells through its redox functional domain
Blood, March 1, 2007; 109(5): 1917 - 1922.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
U. V. Wesley, P. F. Bove, M. Hristova, S. McCarthy, and A. van der Vliet
Airway Epithelial Cell Migration and Wound Repair by ATP-mediated Activation of Dual Oxidase 1
J. Biol. Chem., February 2, 2007; 282(5): 3213 - 3220.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. M. O'Hara, A. Bhattacharyya, R. C. Mifflin, M. F. Smith, K. A. Ryan, K. G.-E. Scott, M. Naganuma, A. Casola, T. Izumi, S. Mitra, et al.
Interleukin-8 Induction by Helicobacter pylori in Gastric Epithelial Cells is Dependent on Apurinic/Apyrimidinic Endonuclease-1/Redox Factor-1
J. Immunol., December 1, 2006; 177(11): 7990 - 7999.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Epidemiol. Biomarkers Prev.Home page
B. A. Sokhansanj and D. M. Wilson III
Estimating the effect of human base excision repair protein variants on the repair of oxidative DNA base damage.
Cancer Epidemiol. Biomarkers Prev., May 1, 2006; 15(5): 1000 - 1008.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. J. Song, I. Steinebrunner, X. Wang, S. C. Stout, and S. J. Roux
Extracellular ATP Induces the Accumulation of Superoxide via NADPH Oxidases in Arabidopsis
Plant Physiology, April 1, 2006; 140(4): 1222 - 1232.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.