Skip Navigation

Nucleic Acids Research 2005 33(14):4544-4552; doi:10.1093/nar/gki766
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (2568K) Freely available
Right arrow Screen PDF (474K) Freely available
Right arrow Supplementary Material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (19)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Yonetani, Y.
Right arrow Articles by Yamazoe, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yonetani, Y.
Right arrow Articles by Yamazoe, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Published online 10 August 2005

© The Author 2005. Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions{at}oupjournals.org

Article

Differential and collaborative actions of Rad51 paralog proteins in cellular response to DNA damage

Yasukazu Yonetani1,2, Helfrid Hochegger2,3, Eiichiro Sonoda2,3, Sayoko Shinya2,4, Hideki Yoshikawa1, Shunichi Takeda2,3 and Mistuyoshi Yamazoe2,3,*

1Department of Orthopedics, Graduate School of Medicine, Osaka University Osaka, Japan 2Department of Radiation Genetics, Faculty of Medicine, Kyoto University Sakyo-ku, Kyoto, Japan 3CREST, Japan Science and Technology Saitama, Japan 4Department of Food and Nutrition, Faculty of Home Economics, Kyoto Women's University Higashiyama-ku, Kyoto, Japan

*To whom correspondence should be addressed. Tel: +81 75 753 4410; Fax: +81 75 753 4419; Email: yamazoe{at}rg.med.kyoto-u.ac.jp

Received May 6, 2005. Revised July 26, 2005. Accepted July 26, 2005.

Metazoan Rad51 plays a central role in homologous DNA recombination, and its activity is controlled by a number of Rad51 cofactors. These include five Rad51 paralogs, Rad51B, Rad51C, Rad51D, XRCC2 and XRCC3. We previously hypothesized that all five paralogs participate collaboratively in repair. However, this idea was challenged by the biochemical identification of two independent complexes composed of either Rad51B/C/D/XRCC2 or Rad51C/XRCC3. To investigate if this biochemical finding is matched by genetic interactions, we made double mutants in either the same complex (rad51b/rad51d) or in both complexes (xrcc3/rad51d). In agreement with the biochemical findings the double deletion involving both complexes had an additive effect on the sensitivity to camptothecin and cisplatin. The double deletion of genes in the same complex, on the other hand, did not further increase the sensitivity to these agents. Conversely, all mutants tested displayed comparatively mild sensitivity to {gamma}-irradiation and attenuated {gamma}-irradiation-induced Rad51 foci formation. Thus, in accord with our previous conclusion, all paralogs appear to collaboratively facilitate Rad51 action. In conclusion, our detailed genetic study reveals a complex interplay between the five Rad51 paralogs and suggests that some of the Rad51 paralogs can separately operate in later step of homologous recombination.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Cell Biol.Home page
M. Otsuki, M. Seki, E. Inoue, A. Yoshimura, G. Kato, S. Yamanouchi, Y.-i. Kawabe, S. Tada, A. Shinohara, J.-i. Komura, et al.
Functional interactions between BLM and XRCC3 in the cell
J. Cell Biol., October 8, 2007; 179(1): 53 - 63.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
S. Kuznetsov, M. Pellegrini, K. Shuda, O. Fernandez-Capetillo, Y. Liu, B. K. Martin, S. Burkett, E. Southon, D. Pati, L. Tessarollo, et al.
RAD51C deficiency in mice results in early prophase I arrest in males and sister chromatid separation at metaphase II in females
J. Cell Biol., February 26, 2007; 176(5): 581 - 592.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Liu, M. Tarsounas, P. O'Regan, and S. C. West
Role of RAD51C and XRCC3 in Genetic Recombination and DNA Repair
J. Biol. Chem., January 19, 2007; 282(3): 1973 - 1979.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
D. Szuts, L. J. Simpson, S. Kabani, M. Yamazoe, and J. E. Sale
Role for RAD18 in Homologous Recombination in DT40 Cells
Mol. Cell. Biol., November 1, 2006; 26(21): 8032 - 8041.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. S. Filippo, P. Chi, M. G. Sehorn, J. Etchin, L. Krejci, and P. Sung
Recombination Mediator and Rad51 Targeting Activities of a Human BRCA2 Polypeptide
J. Biol. Chem., April 28, 2006; 281(17): 11649 - 11657.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. M. Hinz, R. S. Tebbs, P. F. Wilson, P. B. Nham, E. P. Salazar, H. Nagasawa, S. S. Urbin, J. S. Bedford, and L. H. Thompson
Repression of mutagenesis by Rad51D-mediated homologous recombination
Nucleic Acids Res., March 6, 2006; 34(5): 1358 - 1368.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
X. Wu, K. Takenaka, E. Sonoda, H. Hochegger, S. Kawanishi, T. Kawamoto, S. Takeda, and M. Yamazoe
Critical Roles for Polymerase {zeta} in Cellular Tolerance to Nitric Oxide-Induced DNA Damage
Cancer Res., January 15, 2006; 66(2): 748 - 754.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
C. Wiese, J. M. Hinz, R. S. Tebbs, P. B. Nham, S. S. Urbin, D. W. Collins, L. H. Thompson, and D. Schild
Disparate requirements for the Walker A and B ATPase motifs of human RAD51D in homologous recombination.
Nucleic Acids Res., January 1, 2006; 34(9): 2833 - 2843.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.