Skip Navigation

Nucleic Acids Research 2005 33(14):e125; doi:10.1093/nar/gni117
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (830K) Freely available
Right arrow Screen PDF (309K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Egeland, R. D.
Right arrow Articles by Southern, E. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Egeland, R. D.
Right arrow Articles by Southern, E. M.
Related Collections
Right arrow Targeted inhibition of gene function
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Published online 5 August 2005

© The Author 2005. Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions{at}oupjournals.org

Methods Online

Electrochemically directed synthesis of oligonucleotides for DNA microarray fabrication

Ryan D. Egeland1,2,* and Edwin M. Southern1

1Department of Biochemistry, University of Oxford South Parks Road, Oxford OX1 3QU, UK 2Harvard Medical School 260 Longwood Avenue, Boston, MA 02115, USA

*To whom correspondence should be addressed. Tel: +1 857 991 8900; Fax: +1 206 202 4624; Email: ryan{at}egeland.net

Received April 25, 2005. Revised July 13, 2005. Accepted July 13, 2005.

We demonstrate a new method for making oligonucleotide microarrays by synthesis in situ. The method uses conventional DNA synthesis chemistry with an electrochemical deblocking step. Acid is delivered to specific regions on a glass slide, thus allowing nucleotide addition only at chosen sites. The acid is produced by electrochemical oxidation controlled by an array of independent microelectrodes. Deblocking is complete in a few seconds, when competing side-product reactions are minimal. We demonstrate the successful synthesis of 17mers and discrimination of single base pair mismatched hybrids. Features generated in this study are 40 µm wide, with sharply defined edges. The synthetic technique may be applicable to fabrication of other molecular arrays.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 ScienceHome page
M. Beyer, A. Nesterov, I. Block, K. Konig, T. Felgenhauer, S. Fernandez, K. Leibe, G. Torralba, M. Hausmann, U. Trunk, et al.
Combinatorial Synthesis of Peptide Arrays onto a Microchip
Science, December 21, 2007; 318(5858): 1888 - 1888.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. Blair, K. Richmond, M. Rodesch, M. Bassetti, and F. Cerrina
A scalable method for multiplex LED-controlled synthesis of DNA in capillaries
Nucleic Acids Res., September 11, 2006; 34(16): e110 - e110.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
B. Ylstra, P. van den IJssel, B. Carvalho, R. H. Brakenhoff, and G. A. Meijer
BAC to the future! or oligonucleotides: a perspective for micro array comparative genomic hybridization (array CGH)
Nucleic Acids Res., January 26, 2006; 34(2): 445 - 450.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.