Skip Navigation

Nucleic Acids Research 2005 33(15):4673-4682; doi:10.1093/nar/gki774
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (475K) Freely available
Right arrow Screen PDF (353K) Freely available
Right arrow Supplementary Material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (4)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Forner, J.
Right arrow Articles by Binder, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Forner, J.
Right arrow Articles by Binder, S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Published online 17 August 2005

© The Author 2005. Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions{at}oupjournals.org

Article

Distant sequences determine 5' end formation of cox3 transcripts in Arabidopsis thaliana ecotype C24

Joachim Forner, Bärbel Weber, Caterina Wiethölter, Rhonda C. Meyer1 and Stefan Binder*

Molekulare Botanik, Universität Ulm Albert-Einstein-Allee 11, 89069 Ulm, Germany 1MPI für Molekulare Pflanzenphysiologie 14424 Potsdam, Germany

*To whom correspondence should be addressed. Tel: +49 (0) 731 502 2625; Fax: +49 (0) 731 502 2626; Email: stefan.binder{at}biologie.uni-ulm.de

Received July 1, 2005. Revised August 1, 2005. Accepted August 1, 2005.

The genomic environments and the transcripts of the mitochondrial cox3 gene are investigated in three Arabidopsis thaliana ecotypes. While the proximate 5' sequences up to nucleotide position –584, the coding regions and the 3' flanking regions are identical in Columbia (Col), C24 and Landsberg erecta (Ler), genomic variation is detected in regions further upstream. In the mitochondrial DNA of Col, a 1790 bp fragment flanked by a nonanucleotide direct repeat is present beyond position –584 with respect to the ATG. While in Ler only part of this insertion is conserved, this sequence is completely absent in C24, except for a single copy of the nonanucleotide direct repeat. Northern hybridization reveals identical major transcripts in the three ecotypes, but identifies an additional abundant 60 nt larger mRNA species in C24. The extremities of the most abundant mRNA species are identical in the three ecotypes. In C24, an extra major 5' end is abundant. This terminus and the other major 5' ends are located in identical sequence regions. Inspection of Atcox3 transcripts in C24/Col hybrids revealed a female inheritance of the mRNA species with the extra 5' terminus. Thus, a mitochondrially encoded factor determines the generation of an extra 5' mRNA end.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
J. Forner, B. Weber, S. Thuss, S. Wildum, and S. Binder
Mapping of mitochondrial mRNA termini in Arabidopsis thaliana: t-elements contribute to 5' and 3' end formation
Nucleic Acids Res., June 28, 2007; 35(11): 3676 - 3692.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
B. Kusterer, J. Muminovic, H. F. Utz, H.-P. Piepho, S. Barth, M. Heckenberger, R. C. Meyer, T. Altmann, and A. E. Melchinger
Analysis of a Triple Testcross Design With Recombinant Inbred Lines Reveals a Significant Role of Epistasis in Heterosis for Biomass-Related Traits in Arabidopsis
Genetics, April 1, 2007; 175(4): 2009 - 2017.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
V. Shedge, M. Arrieta-Montiel, A. C. Christensen, and S. A. Mackenzie
Plant Mitochondrial Recombination Surveillance Requires Unusual RecA and MutS Homologs
PLANT CELL, April 1, 2007; 19(4): 1251 - 1264.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
V. Zaegel, B. Guermann, M. Le Ret, C. Andres, D. Meyer, M. Erhardt, J. Canaday, J. M. Gualberto, and P. Imbault
The Plant-Specific ssDNA Binding Protein OSB1 Is Involved in the Stoichiometric Transmission of Mitochondrial DNA in Arabidopsis
PLANT CELL, December 1, 2006; 18(12): 3548 - 3563.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.