Skip Navigation

Nucleic Acids Research 2005 33(15):4725-4739; doi:10.1093/nar/gki783
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (4051K) Freely available
Right arrow Screen PDF (511K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (4)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Dai, H.
Right arrow Articles by Chiang, K.-S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dai, H.
Right arrow Articles by Chiang, K.-S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Published online 22 August 2005

© The Author 2005. Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions{at}oupjournals.org

Article

Structural and functional characterizations of mung bean mitochondrial nucleoids

Hwa Dai*, Yih-Shan Lo, Alexandra Litvinchuk, Yuh-Tai Wang, Wann-Neng Jane, Lin-June Hsiao and Kwen-Sheng Chiang1

Institute of Plant and Microbial Biology, Academia Sinica Taipei, Taiwan 11509, ROC 1Department of Molecular Genetics and Cell Biology, The University of Chicago Chicago, IL 60637, USA

*To whom correspondence should be addressed. Tel: +886 2 27891498; Fax: +886 2 27838609; Email: bodaihwa{at}gate.sinica.edu.tw

Received May 3, 2005. Revised August 3, 2005. Accepted August 3, 2005.

Mitochondrial nucleoids isolated from mung bean seedlings exhibited a chromatin-like structure associated with a membrane component. A similar structure, which underwent discrete changes during cotyledon development, was identified in situ. Isolated nucleoids consisted of essentially the same phospholipids, including cardiolipin, as whole mitochondria and proteins of inner- and outer-mitochondrial-membrane origin. Actin was consistently found with mitochondrial nucleoids prepared with different detergent concentrations. Formaldehyde cross-linking of cytochalasin B- and proteinase K-treated mitochondria further revealed that actin was associated with DNA in nucleoids. Mitochondrial nucleoids were self-sufficient in directing DNA synthesis in vitro in a pattern mimicking mtDNA synthesis in isolated mitochondria. In pulse-field gel electrophoresis, newly synthesized mtDNA separated into two major components, well-bound and fast-moving forms. Nucleoids DNA synthesis was resistant to aphidicolin but sensitive to N-ethylmaleimide, which indicates that a {gamma}-type DNA polymerase was responsible for this activity. Mitochondrial nucleoids were capable of self-directed RNA transcription in a non-random fashion in vitro. Consistent with and complementary to results from fungi and human cells done mostly in situ, our present work helps to establish the important paradigm that mitochondrial nucleoids in eukaryotes are more than mere mtDNA compaction and segregation entities but are centers of mtDNA maintenance and expression.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
X. J. Chen, X. Wang, and R. A. Butow
Yeast aconitase binds and provides metabolically coupled protection to mitochondrial DNA
PNAS, August 21, 2007; 104(34): 13738 - 13743.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
D. T. Johnson, R. A. Harris, S. French, P. V. Blair, J. You, K. G. Bemis, M. Wang, and R. S. Balaban
Tissue heterogeneity of the mammalian mitochondrial proteome
Am J Physiol Cell Physiol, February 1, 2007; 292(2): C689 - C697.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.