Published online 6 September 2005
Article |
An evolutionary intra-molecular shift in the preferred U3 snoRNA binding site on pre-ribosomal RNA
Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Division of Biology and Medicine Providence, RI 02912, USA
*To whom correspondence should be addressed. Tel: +1 401 863 2359; Fax: +1 401 863 1348; Email: Susan_Gerbi{at}Brown.edu
Received May 17, 2005. Revised July 15, 2005. Accepted August 18, 2005.
Correct docking of U3 small nucleolar RNA (snoRNA) on pre-ribosomal RNA (pre-rRNA) is essential for rRNA processing to produce 18S rRNA. In this report, we have used Xenopus oocytes to characterize the structural requirements of the U3 snoRNA 3'-hinge interaction with region E1 of the external transcribed spacer (ETS) of pre-rRNA. This interaction is crucial for docking to initiate rRNA processing. 18S rRNA production was inhibited when fewer than 6 of the 8 bp of the U3 3'–hinge complex with the ETS could form; moreover, base pairing involving the right side of the 3'-hinge was more important than the left. Increasing the length of the U3 hinge–ETS interaction by 9 bp impaired rRNA processing. Formation of 18S rRNA was also inhibited by swapping the U3 5'- and 3'-hinge interactions with the ETS or by shifting the base pairing of the U3 3'-hinge to the sequence directly adjacent to ETS region E1. However, 18S rRNA production was partially restored by a compensatory shift that allowed the sequence adjacent to the U3 3'-hinge to pair with the eight bases directly adjacent to ETS region E1. The results suggest that the geometry of the U3 snoRNA interaction with the ETS is critical for rRNA processing.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  T. Kent, Y. R. Lapik, and D. G. Pestov The 5' external transcribed spacer in mouse ribosomal RNA contains two cleavage sites RNA, January 1, 2009; 15(1): 14 - 20. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Clery, V. Senty-Segault, F. Leclerc, H. A. Raue, and C. Branlant Analysis of Sequence and Structural Features That Identify the B/C Motif of U3 Small Nucleolar RNA as the Recognition Site for the Snu13p-Rrp9p Protein Pair Mol. Cell. Biol., February 15, 2007; 27(4): 1191 - 1206. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Bax, H. A. Raue, and J. C. Vos Slx9p facilitates efficient ITS1 processing of pre-rRNA in Saccharomyces cerevisiae RNA, November 1, 2006; 12(11): 2005 - 2013. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Bax, H. R. Vos, H. A. Raue, and J. C. Vos Saccharomyces cerevisiae Sof1p Associates with 35S Pre-rRNA Independent from U3 snoRNA and Rrp5p Eukaryot. Cell, March 1, 2006; 5(3): 427 - 434. [Abstract] [Full Text] [PDF]
|
|