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Nucleic Acids Research 2005 33(16):5181-5189; doi:10.1093/nar/gki827
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Published online 9 September 2005

© The Author 2005. Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions{at}oxfordjournals.org

Molecular Biology

An Sp1/KLF binding site is important for the activity of a Polycomb group response element from the Drosophila engrailed gene

J. Lesley Brown, Daniel J. Grau, Sarah K. DeVido and Judith A. Kassis*

Laboratory of Molecular Genetics, National Institutes of Child Health and Human Development, National Institutes of Health Bethesda, MD, 20892, USA

*To whom correspondence should be addressed. Tel: +1 301 496 7879; Fax: +1 301 496 0243; Email: jkassis{at}mail.nih.gov

Received June 13, 2005. Revised August 23, 2005. Accepted August 23, 2005.

Polycomb-group response elements (PREs) are DNA elements through which the Polycomb-group (PcG) of transcriptional repressors act. Many of the PcG proteins are associated with two protein complexes that repress gene expression by modifying chromatin. Both of these protein complexes specifically associate with PREs in vivo, however, it is not known how they are recruited or held at the PRE. PREs are complex elements, made up of binding sites for many proteins. Our laboratory has been working to define all the sequences and DNA binding proteins required for the activity of a 181 bp PRE from the Drosophila engrailed gene. Here we show that one of the sites necessary for PRE activity, Site 2, can be bound by members of the Sp1/KLF family of zinc finger proteins. There are 10 Sp1/KLF family members in Drosophila, and nine of them bind to Site 2. We derive a consensus binding site for the Sp1/KLF Drosophila family members and show that this consensus sequence is present in most of the molecularly characterized PREs. These data suggest that one or more Sp1/KLF family members play a role in PRE function in Drosophila.

Present address: Daniel J. Grau, Department of Genetics, Harvard Medical School, Boston, MA 02115, USA


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