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Nucleic Acids Research 2005 33(17):5404-5414; doi:10.1093/nar/gki854
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Published online 22 September 2005

© The Author 2005. Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions{at}oxfordjournals.org

Article

The genes encoding Arabidopsis ORC subunits are E2F targets and the two ORC1 genes are differently expressed in proliferating and endoreplicating cells

Sara Diaz-Trivino, María del Mar Castellano, María de la Paz Sanchez, Elena Ramirez-Parra, Bénédicte Desvoyes and Crisanto Gutierrez*

Centro de Biologia Molecular ‘Severo Ochoa’, Consejo Superior de Investigaciones Científicas and Universidad Autónoma de Madrid Cantoblanco, 28049 Madrid, Spain

*To whom correspondence should be addressed. Tel: +34 91 497 8430; Fax: +34 91 4974799; Email: cgutierrez{at}cbm.uam.es

Received June 14, 2005. Revised September 6, 2005. Accepted September 6, 2005.

Initiation of eukaryotic DNA replication depends on the function of pre-replication complexes (pre-RC), one of its key component being the six subunits origin recognition complex (ORC). In spite of a significant degree of conservation among ORC proteins from different eukaryotic sources, the regulation of their availability varies considerably in different model systems and cell types. Here, we show that the six ORC genes of Arabidopsis thaliana are regulated at the transcriptional level during cell cycle and development. We found that Arabidopsis ORC genes, except AtORC5, contain binding sites for the E2F family of transcription factors. Expression of AtORC genes containing E2F binding sites peaks at the G1/S-phase. Analysis of AtORC gene expression in plants with reduced E2F activity, obtained by expressing a dominant negative version of DP, the E2F heterodimerization partner, and with increased E2F activity, obtained by inactivation of the retinoblastoma protein, led us to conclude that all AtORC genes, except AtORC5 are E2F targets. Interestingly, Arabidopsis contains two AtORC1 (a and b) genes, highly conserved at the amino acid level but with unrelated promoter sequences. AtORC1b expression is restricted to proliferating cells. However, AtORC1a is preferentially expressed in endoreplicating cells based on our analysis in endoreplicating tissues and in a mutant with altered endocycle pattern. This suggests a differential expression of the two ORC1 genes in Arabidopsis.

DDBJ/EMBL/GenBank accession nos+

+AJ421410, AJ426477, CAE01428–CAE01430


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