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Nucleic Acids Research 2005 33(17):e145; doi:10.1093/nar/gni153
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Published online 4 October 2005

© The Author 2005. Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions{at}oxfordjournals.org

Methods Online

Expressible molecular colonies

Timur R. Samatov, Helena V. Chetverina and Alexander B. Chetverin*

Institute of Protein Research, Russian Academy of Sciences Pushchino, Moscow Region 142290, Russia

*To whom correspondence should be addressed. Tel/Fax: +7 095 632 7871; Email: alexch{at}vega.protres.ru

Received July 20, 2005. Revised September 19, 2005. Accepted September 19, 2005.

Carrying out polymerase chain reaction in a gel layer generates a 2-D pattern of DNA colonies comprising pure genetic clones. Here we demonstrate that transcription, translation and protein folding can be performed in the same gel. The resulting nucleoprotein colonies mimic living cells by serving as compartments in which the synthesized RNAs and proteins co-localize with their templates. Yet, due to the absence of penetration barriers, such a molecular colony display allows cloned genes to be directly tested for the encoded functions. Now, the results imply that virtually any manipulations with genes and their expression products can be accomplished in vitro.


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