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Nucleic Acids Research 2005 33(2):776-785; doi:10.1093/nar/gki221
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Published online 1 February 2005

© The Author 2005. Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions{at}oupjournals.org.

Article

In vivo RNA localization of I factor, a non-LTR retrotransposon, requires a cis-acting signal in ORF2 and ORF1 protein

Maria del Carmen Seleme, Olivier Disson, Stéphanie Robin, Christine Brun, Danielle Teninges* and Alain Bucheton

Institut de Génétique Humaine, C.N.R.S. 141 rue de la Cardonille, 34396 Montpellier, France

*To whom correspondence should be addressed. Tel: +33 0 4 99 61 99 47; Email: Danielle.Teninges{at}igh.cnrs.fr

Received October 27, 2004. Revised January 13, 2005. Accepted January 13, 2005.

According to the current model of non-LTR retrotransposon (NLR) mobilization, co-expression of the RNA transposition intermediate, and the proteins it encodes (ORF1p and ORF2p), is a requisite for the formation of cytoplasmic ribonucleoprotein complexes which contain necessary elements to complete a retrotransposition cycle later in the nucleus. To understand these early processes of NLR mobilization, here we analyzed in vivo the protein and RNA expression patterns of the I factor, a model NLR in Drosophila. We show that ORF1p and I factor RNA, specifically produced during transposition, are co-expressed and tightly co-localize with a specific pattern (Loc+) exclusively in the cytoplasm of germ cells permissive for retrotransposition. Using an ORF2 mutated I factor, we show that ORF2p plays no role in the Loc+ patterning. With deletion derivatives of an I factor we define an RNA localization signal required to display the Loc+ pattern. Finally, by complementation experiments we show that ORF1p is necessary for the efficient localization of I factor RNA. Our data suggest that ORF1p is involved in proper folding and stabilization of I factor RNA for efficient targeting, through Loc+ patterning, to the nuclear neighborhood where downstream steps of the retrotransposition process occur.

Present addresses: Maria del Carmen Seleme, Department of Genetics, The University of Pennsylvania Medical School, Philadelphia, PA 19104, USA

Olivier Disson, Institut de Génétique Moléculaire de Montpellier, C.N.R.S., 1919 Route de Mende 34090 Montpellier, France


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