Published online 31 January 2005
Methods Online |
An oligonucleotide microarray for microRNA expression analysis based on labeling RNA with quantum dot and nanogold probe
Key Laboratory of Proteomics 320 Yue-Yang Road, Shanghai 200031, China 1 State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences 320 Yue-Yang Road, Shanghai 200031, China 2 Department of Oral Biology, College of Dentistry, University of Illinois at Chicago Chicago, IL 60612, USA
*To whom correspondence should be addressed. Tel: +86 21 5492 1168; Fax: +86 21 5492 1011; Email: kcruan{at}sibs.ac.cn
Received August 24, 2004. Revised December 3, 2004. Accepted January 10, 2005.
MicroRNAs (miRNAs) play important regulatory roles in animals and plants by targeting mRNAs for cleavage or translational repression. They have diverse expression patterns and might regulate various developmental and physiological processes. Profiling miRNA expression is very helpful for studying biological functions of miRNAs. We report a novel miRNA profiling microarray, in which miRNAs were directly labeled at the 3' terminus with biotin and hybridized with complementary oligo-DNA probes immobilized on glass slides, and subsequently detected by measuring fluorescence of quantum dots labeled with streptavidin bound to miRNAs through streptavidin–biotin interaction. The detection limit of this microarray for miRNA was 
0.4 fmol, and the detection dynamic range spanned about 2 orders of magnitude. We made a model microarray to profile 11 miRNAs from leaf and root of rice (Oryza sativa L. ssp. indica) seedlings. The analysis results of the miRNAs had a good reproducibility and were consistent with the northern blot result. To avoid using high-cost detection equipment, colorimetric detection, a method based on nanogold probe coupled with silver enhancement, was also successfully introduced into miRNA profiling microarray detection.
Correspondence may also be addressed to You-Xin Jin. Tel: +86 21 5492 1222; Fax: +86 21 5492 1011; Email: yxjin{at}sunm.shcnc.ac.cn
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  U. Lakshmipathy and R. P. Hart Concise Review: MicroRNA Expression in Multipotent Mesenchymal Stromal Cells Stem Cells, February 1, 2008; 26(2): 356 - 363. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Tang, J. Gal, X. Zhuang, W. Wang, H. Zhu, and G. Tang A simple array platform for microRNA analysis and its application in mouse tissues RNA, October 1, 2007; 13(10): 1803 - 1822. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Beuvink, F. A. Kolb, W. Budach, A. Garnier, J. Lange, F. Natt, U. Dengler, J. Hall, W. Filipowicz, and J. Weiler A novel microarray approach reveals new tissue-specific signatures of known and predicted mammalian microRNAs Nucleic Acids Res., April 1, 2007; 35(7): e52 - e52. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Wang, R. A. Ach, and B. Curry Direct and sensitive miRNA profiling from low-input total RNA RNA, January 1, 2007; 13(1): 151 - 159. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P Pallante, R Visone, M Ferracin, A Ferraro, M T Berlingieri, G Troncone, G Chiappetta, C G Liu, M Santoro, M Negrini, et al. MicroRNA deregulation in human thyroid papillary carcinomas. Endocr. Relat. Cancer, June 1, 2006; 13(2): 497 - 508. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Tang, P. Hajkova, S. C. Barton, K. Lao, and M. A. Surani MicroRNA expression profiling of single whole embryonic stem cells Nucleic Acids Res., January 24, 2006; 34(2): e9 - e9. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E.-Y. Kim, J. Stanton, R. A. Vega, K. J. Kunstman, C. A. Mirkin, and S. M. Wolinsky A real-time PCR-based method for determining the surface coverage of thiol-capped oligonucleotides bound onto gold nanoparticles. Nucleic Acids Res., January 1, 2006; 34(7): e54 - e54. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Chan, T. Yuen, F. Ruf, J. Gonzalez-Maeso, and S. C. Sealfon Method for multiplex cellular detection of mRNAs using quantum dot fluorescent in situ hybridization Nucleic Acids Res., October 13, 2005; 33(18): e161 - e161. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. V. Iorio, M. Ferracin, C.-G. Liu, A. Veronese, R. Spizzo, S. Sabbioni, E. Magri, M. Pedriali, M. Fabbri, M. Campiglio, et al. MicroRNA Gene Expression Deregulation in Human Breast Cancer Cancer Res., August 15, 2005; 65(16): 7065 - 7070. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Axtell and D. P. Bartel Antiquity of MicroRNAs and Their Targets in Land Plants PLANT CELL, June 1, 2005; 17(6): 1658 - 1673. [Abstract] [Full Text] [PDF]
|
|