Skip Navigation

Nucleic Acids Research 2005 33(20):6566-6578; doi:10.1093/nar/gki965
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (1502K) Freely available
Right arrow Screen PDF (1510K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (35)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Vandewalle, C.
Right arrow Articles by Berx, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vandewalle, C.
Right arrow Articles by Berx, G.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Published online 24 November 2005

© The Author 2005. Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions{at}oxfordjournals.org

Article

SIP1/ZEB2 induces EMT by repressing genes of different epithelial cell–cell junctions

Cindy Vandewalle, Joke Comijn, Bram De Craene, Petra Vermassen, Erik Bruyneel1, Henriette Andersen2, Eugene Tulchinsky3, Frans Van Roy4 and Geert Berx*

Unit of Molecular and Cellular Oncology, Department for Molecular Biomedical Research, VIB-Ghent University Belgium 1Laboratory of Experimental Cancerology, Department of Radiotherapy and Nuclear Medicine, Ghent University Belgium 2Department of Molecular Cancer Biology, Danish Cancer Society Denmark 3Department of Cancer Studies and Molecular Medicine, University of Leicester UK 4Molecular Cell Biology Unit, Department for Molecular Biomedical Research, VIB-Ghent University Belgium

*To whom correspondence should be addressed at Department for Molecular Biomedical Research, VIB-Ghent University, Technologiepark 927, 9052 Ghent (Zwijnaarde), Belgium. Tel: +32.0 9.3313740; Fax: +32.0 9.3313609; Email: geert.berx{at}dmbr.UGent.be

Received August 12, 2005. Revised November 2, 2005. Accepted November 2, 2005.

SIP1/ZEB2 is a member of the {delta}EF-1 family of two-handed zinc finger nuclear factors. The expression of these transcription factors is associated with epithelial mesenchymal transitions (EMT) during development. SIP1 is also expressed in some breast cancer cell lines and was detected in intestinal gastric carcinomas, where its expression is inversely correlated with that of E-cadherin. Here, we show that expression of SIP1 in human epithelial cells results in a clear morphological change from an epithelial to a mesenchymal phenotype. Induction of this epithelial dedifferentiation was accompanied by repression of several cell junctional proteins, with concomitant repression of their mRNA levels. Besides E-cadherin, other genes coding for crucial proteins of tight junctions, desmosomes and gap junctions were found to be transcriptionally regulated by the transcriptional repressor SIP1. Moreover, study of the promoter regions of selected genes by luciferase reporter assays and chromatin immunoprecipitation shows that repression is directly mediated by SIP1. These data indicate that, during epithelial dedifferentiation, SIP1 represses in a coordinated manner the transcription of genes coding for junctional proteins contributing to the dedifferentiated state; this repression occurs by a general mechanism mediated by Smad Interacting Protein 1 (SIP1)-binding sites.

The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
W.-Y. Huang, Z.-G. Li, H. Rus, X. Wang, P. A. Jose, and S.-Y. Chen
RGC-32 Mediates Transforming Growth Factor-{beta}-induced Epithelial-Mesenchymal Transition in Human Renal Proximal Tubular Cells
J. Biol. Chem., April 3, 2009; 284(14): 9426 - 9432.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
V. R. Sobrado, G. Moreno-Bueno, E. Cubillo, L. J. Holt, M. A. Nieto, F. Portillo, and A. Cano
The class I bHLH factors E2-2A and E2-2B regulate EMT
J. Cell Sci., April 1, 2009; 122(7): 1014 - 1024.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
P. Gandellini, M. Folini, N. Longoni, M. Pennati, M. Binda, M. Colecchia, R. Salvioni, R. Supino, R. Moretti, P. Limonta, et al.
miR-205 Exerts Tumor-Suppressive Functions in Human Prostate through Down-regulation of Protein Kinase C{varepsilon}
Cancer Res., March 15, 2009; 69(6): 2287 - 2295.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. V. Shah, A. Muralidharan, M. Gokulgandhi, K. Soan, and S. Thomas
Cadherin Switching and Activation of {beta}-Catenin Signaling Underlie Proinvasive Actions of Calcitonin-Calcitonin Receptor Axis in Prostate Cancer
J. Biol. Chem., January 9, 2009; 284(2): 1018 - 1030.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
C. P. Bracken, P. A. Gregory, N. Kolesnikoff, A. G. Bert, J. Wang, M. F. Shannon, and G. J. Goodall
A Double-Negative Feedback Loop between ZEB1-SIP1 and the microRNA-200 Family Regulates Epithelial-Mesenchymal Transition
Cancer Res., October 1, 2008; 68(19): 7846 - 7854.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
M. Beltran, I. Puig, C. Pena, J. M. Garcia, A. B. Alvarez, R. Pena, F. Bonilla, and A. G. de Herreros
A natural antisense transcript regulates Zeb2/Sip1 gene expression during Snail1-induced epithelial-mesenchymal transition
Genes & Dev., March 15, 2008; 22(6): 756 - 769.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
L. Ulianich, C. Garbi, A. S. Treglia, D. Punzi, C. Miele, G. A. Raciti, F. Beguinot, E. Consiglio, and B. Di Jeso
ER stress is associated with dedifferentiation and an epithelial-to-mesenchymal transition-like phenotype in PC Cl3 thyroid cells
J. Cell Sci., February 15, 2008; 121(4): 477 - 486.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. L. Criswell and C. L. Arteaga
Modulation of NF{kappa}B Activity and E-cadherin by the Type III Transforming Growth Factor beta Receptor Regulates Cell Growth and Motility
J. Biol. Chem., November 2, 2007; 282(44): 32491 - 32500.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
J. Mejlvang, M. Kriajevska, C. Vandewalle, T. Chernova, A. E. Sayan, G. Berx, J. K. Mellon, and E. Tulchinsky
Direct Repression of Cyclin D1 by SIP1 Attenuates Cell Cycle Progression in Cells Undergoing an Epithelial Mesenchymal Transition
Mol. Biol. Cell, November 1, 2007; 18(11): 4615 - 4624.
[Abstract] [Full Text] [PDF]

Home page
 Ann OncolHome page
M. Mimeault and S. K. Batra
Interplay of distinct growth factors during epithelial mesenchymal transition of cancer progenitor cells and molecular targeting as novel cancer therapies
Ann. Onc., October 1, 2007; 18(10): 1605 - 1619.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
X. Wang, M. Zheng, G. Liu, W. Xia, P. J. McKeown-Longo, M.-C. Hung, and J. Zhao
Kruppel-Like Factor 8 Induces Epithelial to Mesenchymal Transition and Epithelial Cell Invasion
Cancer Res., August 1, 2007; 67(15): 7184 - 7193.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. A. Mani, J. Yang, M. Brooks, G. Schwaninger, A. Zhou, N. Miura, J. L. Kutok, K. Hartwell, A. L. Richardson, and R. A. Weinberg
Mesenchyme Forkhead 1 (FOXC2) plays a key role in metastasis and is associated with aggressive basal-like breast cancers
PNAS, June 12, 2007; 104(24): 10069 - 10074.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
A. J. Evans, R. C. Russell, O. Roche, T. N. Burry, J. E. Fish, V. W. K. Chow, W. Y. Kim, A. Saravanan, M. A. Maynard, M. L. Gervais, et al.
VHL Promotes E2 Box-Dependent E-Cadherin Transcription by HIF-Mediated Regulation of SIP1 and Snail
Mol. Cell. Biol., January 1, 2007; 27(1): 157 - 169.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
M. Takkunen, R. Grenman, M. Hukkanen, M. Korhonen, A. Garcia de Herreros, and I. Virtanen
Snail-dependent and -independent Epithelial-Mesenchymal Transition in Oral Squamous Carcinoma Cells
J. Histochem. Cytochem., November 1, 2006; 54(11): 1263 - 1275.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.