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Nucleic Acids Research 2005 33(21):6688-6693; doi:10.1093/nar/gki980
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Published online 27 November 2005

© The Author 2005. Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions{at}oxfordjournals.org

Article

The genetic map and comparative analysis with the physical map of Trypanosoma brucei

Annette MacLeod1,*, Alison Tweedie1, Sarah McLellan2, Sonya Taylor2, Neil Hall3, Matthew Berriman3, Najib M. El-Sayed4, Michelle Hope1, C. Michael R. Turner1,2 and Andy Tait1

1Wellcome Centre for Molecular Parasitology, Anderson College Complex, University of Glasgow 56 Dumbarton Road, Glasgow G11 6NU, UK 2Division of Infection and Immunity, Institute of Biomedical and Life Sciences Joseph Black Building University of Glasgow G12 8QQ UK 3Wellcome Trust Sanger Institute Wellcome Trust Genome Campus, Hinxton CB10 1SA 4The Institute for Genomic Research 9712 Medical Center Drive, Rockville, MD 20850, USA

*To whom correspondence should be addressed. Tel: 0 141 330 3579; Fax: 0 141 330 5422; Email: gvwa08{at}udcf.gla.ac.uk

Received September 5, 2005. Revised October 20, 2005. Accepted November 8, 2005.

Trypanosoma brucei is the causative agent of African sleeping sickness in humans and contributes to the debilitating disease ‘Nagana’ in cattle. To date we know little about the genes that determine drug resistance, host specificity, pathogenesis and virulence in these parasites. The availability of the complete genome sequence and the ability of the parasite to undergo genetic exchange have allowed genetic investigations into this parasite and here we report the first genetic map of T.brucei for the genome reference stock TREU 927, comprising of 182 markers and 11 major linkage groups, that correspond to the 11 previously identified chromosomes. The genetic map provides 90% probability of a marker being 11 cM from any given locus. Its comparison to the available physical map has revealed the average physical size of a recombination unit to be 15.6 Kb/cM. The genetic map coupled with the genome sequence and the ability to undertake crosses presents a new approach to identifying genes relevant to the disease and its prevention in this important pathogen through forward genetic analysis and positional cloning.

Present addresses: Neil Hall, TIGR, 9712 Medical Center Drive Rockville, MD 20850, USA

Michelle Hope, CSIRO Livestock Industries, MAGC, Research Road, St Lucia, Brisbane, Australia


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