Skip Navigation

Nucleic Acids Research 2005 33(22):e194; doi:10.1093/nar/gni185
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (1060K) Freely available
Right arrow Screen PDF (247K) Freely available
Right arrow Supplementary Material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Gu, A.-D.
Right arrow Articles by Qu, L.-H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gu, A.-D.
Right arrow Articles by Qu, L.-H.
Related Collections
Right arrow RNA characterisation and manipulation
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Published online 15 December 2005

© The Author 2005. Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions{at}oxfordjournals.org

Methods Online

A novel experimental approach for systematic identification of box H/ACA snoRNAs from eukaryotes

Ai-Di Gu, Hui Zhou, Chun-Hong Yu and Liang-Hu Qu*

Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory for Biocontrol, Zhongshan University Guangzhou 510275, People's Republic of China

*To whom correspondence should be addressed. Tel: +86 20 84112399; Fax: +86 20 84036551; Email: lsbrc04{at}zsu.edu.cn

Received October 27, 2005. Revised November 18, 2005. Accepted November 18, 2005.

Box H/ACA snoRNAs represent an abundant group of small non-coding RNAs mainly involved in the pseudouridylation of rRNAs and/or snRNAs in eukaryotes and Archaea. In this study, we describe a novel experimental method for systematic identification of box H/ACA snoRNAs from eukaryotes. In the specialized cDNA libraries constructed by this method with total cellular RNAs from human blood cells, the high efficiency of cloning for diverse box H/ACA snoRNAs was achieved and seven novel species of this snoRNA family were identified from human for the first time. Furthermore, the novel method has been successfully applied for the identification of the box H/ACA snoRNAs from Drosophila and the fission yeast, demonstrating a powerful ability for systematic analysis of box H/ACA snoRNAs in a broad spectrum of eukaryotes.

The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
D. Piekna-Przybylska, W. A. Decatur, and M. J. Fournier
The 3D rRNA modification maps database: with interactive tools for ribosome analysis
Nucleic Acids Res., January 11, 2008; 36(suppl_1): D178 - D183.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
K. Chakrabarti, M. Pearson, L. Grate, T. Sterne-Weiler, J. Deans, J. P. Donohue, and M. Ares Jr
Structural RNAs of known and unknown function identified in malaria parasites by comparative genomics and RNA analysis
RNA, November 1, 2007; 13(11): 1923 - 1939.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
Y. L. a. S. Li
Genome-wide analyses of retrogenes derived from the human box H/ACA snoRNAs
Nucleic Acids Res., January 28, 2007; 35(2): 559 - 571.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J.-H. Yang, X.-C. Zhang, Z.-P. Huang, H. Zhou, M.-B. Huang, S. Zhang, Y.-Q. Chen, and L.-H. Qu
snoSeeker: an advanced computational package for screening of guide and orphan snoRNA genes in the human genome
Nucleic Acids Res., October 6, 2006; (2006) gkl672v3.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.