Skip Navigation

Nucleic Acids Research 2005 33(5):1618-1627; doi:10.1093/nar/gki308
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (478K) Freely available
Right arrow Screen PDF (486K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (22)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Erlacher, M. D.
Right arrow Articles by Polacek, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Erlacher, M. D.
Right arrow Articles by Polacek, N.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Published online 14 March 2005

© The Author 2005. Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions{at}oupjournals.org

Article

Chemical engineering of the peptidyl transferase center reveals an important role of the 2'-hydroxyl group of A2451

Matthias D. Erlacher, Kathrin Lang1, Nisha Shankaran2, Brigitte Wotzel, Alexander Hüttenhofer, Ronald Micura1, Alexander S. Mankin2 and Norbert Polacek*

Innsbruck Biocenter, Division of Genomics and RNomics—Innsbruck Medical University Fritz-Pregl Strasse 3, 6020 Innsbruck, Austria 1Center for Molecular Biosciences Innsbruck (CMBI), Institute of Organic Chemistry, Leopold Franzens University Innrain 52a, 6020 Innsbruck, Austria 2Center for Pharmaceutical Biotechnology—M/C 870, University of Illinois at Chicago 900 S. Ashland Avenue, Chicago, IL 60607, USA

*To whom correspondence should be addressed. Tel: +43 512 507 3384; Fax: +43 512 507 9880; Email: norbert.polacek{at}uibk.ac.at

Received January 6, 2005. Revised February 28, 2005. Accepted February 28, 2005.

The main enzymatic reaction of the large ribosomal subunit is peptide bond formation. Ribosome crystallography showed that A2451 of 23S rRNA makes the closest approach to the attacking amino group of aminoacyl-tRNA. Mutations of A2451 had relatively small effects on transpeptidation and failed to unequivocally identify the crucial functional group(s). Here, we employed an in vitro reconstitution system for chemical engineering the peptidyl transferase center by introducing non-natural nucleosides at position A2451. This allowed us to investigate the peptidyl transfer reaction performed by a ribosome that contained a modified nucleoside at the active site. The main finding is that ribosomes carrying a 2'-deoxyribose at A2451 showed a compromised peptidyl transferase activity. In variance, adenine base modifications and even the removal of the entire nucleobase at A2451 had only little impact on peptide bond formation, as long as the 2'-hydroxyl was present. This implicates a functional or structural role of the 2'-hydroxyl group at A2451 for transpeptidation.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
M. Amort, B. Wotzel, K. Bakowska-Zywicka, M. D. Erlacher, R. Micura, and N. Polacek
An intact ribose moiety at A2602 of 23S rRNA is key to trigger peptidyl-tRNA hydrolysis during translation termination
Nucleic Acids Res., August 1, 2007; 35(15): 5130 - 5140.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
N. S. Sato, N. Hirabayashi, I. Agmon, A. Yonath, and T. Suzuki
Comprehensive genetic selection revealed essential bases in the peptidyl-transferase center
PNAS, October 17, 2006; 103(42): 15386 - 15391.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
M. Selmer, C. M. Dunham, F. V. Murphy IV, A. Weixlbaumer, S. Petry, A. C. Kelley, J. R. Weir, and V. Ramakrishnan
Structure of the 70S Ribosome Complexed with mRNA and tRNA
Science, September 29, 2006; 313(5795): 1935 - 1942.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Beringer, C. Bruell, L. Xiong, P. Pfister, P. Bieling, V. I. Katunin, A. S. Mankin, E. C. Bottger, and M. V. Rodnina
Essential Mechanisms in the Catalysis of Peptide Bond Formation on the Ribosome
J. Biol. Chem., October 28, 2005; 280(43): 36065 - 36072.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Trobro and J. Aqvist
Mechanism of peptide bond synthesis on the ribosome
PNAS, August 30, 2005; 102(35): 12395 - 12400.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.