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Nucleic Acids Research 2005 33(5):e46; doi:10.1093/nar/gni046
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Published online 10 March 2005

© The Author 2005. Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions{at}oupjournals.org

Methods Online

Methylation profiles of genes utilizing newly developed CpG island methylation microarray on colorectal cancer patients

Naoki Kimura, Takeshi Nagasaka1, Jun Murakami2, Hiromi Sasamoto1, Masahiro Murakami, Noriaki Tanaka1 and Nagahide Matsubara1,*

Research and Development Center, Nisshinbo Industries Inc. 1-2-3 Ohnodai, Midori-ku, Chiba-shi, Chiba 267-0056, Japan 1Department of Gastroenterological Surgery and Surgical Oncology, Okayama University Graduate School of Medicine and Dentistry 2-5-1 Shikata-cho, Okayama 700-8558, Japan 2Department of Dental Radiology, Okayama University Graduate School of Medicine and Dentistry 2-5-1 Shikata-cho, Okayama 700-8558, Japan

*To whom correspondence should be addressed. Tel: +81 86 235 7257; Fax: +81 86 221 8775; Email: nagamb{at}cc.okayama-u.ac.jp

Received November 11, 2004. Revised January 7, 2005. Accepted February 17, 2005.

Aberrant methylation of DNA has been shown to play an important role in a variety of human cancers, developmental disorders and aging. Hence, aberrant methylation patterns in genes can be a molecular marker for such conditions. Therefore, a reliable but uncomplicated method to detect DNA methylation is preferred, not merely for research purposes but for daily clinical practice. To achieve these aims, we have established a precise system to identify DNA methylation patterns based on an oligonucleotide microarray technology. Our microarray method has an advantage over conventional methods and is unique because it allows the precise measurement of the methylation patterns within a target region. Our simple signal detection system depends on using an avidin–biotinylated peroxidase complex and does not require an expensive laser scanner or hazardous radioisotope. In this study, we applied our technique to detect promoter methylation status of O6-methylguanine-DNA methyltransferase (MGMT) gene. Our easy-handling technology provided reproducible and precise measurement of methylated CpGs in MGMT promoter and, thus, our method may bring about a potential evolution in the handling of a variety of high-throughput DNA methylation analyses for clinical purposes.


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