Skip Navigation

Nucleic Acids Research 2005 33(7):2181-2191; doi:10.1093/nar/gki513
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (1087K) Freely available
Right arrow Screen PDF (330K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (8)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Nakano, T.
Right arrow Articles by Ide, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nakano, T.
Right arrow Articles by Ide, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Published online 14 April 2005

© The Author 2005. Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions{at}oupjournals.org

Article

Repair activity of base and nucleotide excision repair enzymes for guanine lesions induced by nitrosative stress

Toshiaki Nakano, Atsushi Katafuchi, Ryoko Shimizu, Hiroaki Terato, Toshinori Suzuki1, Hiroshi Tauchi2, Keisuke Makino3, Milan Skorvaga4, Bennett Van Houten4 and Hiroshi Ide*

Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University Higashi-Hiroshima 739-8526, Japan 1Department of Biological Pharmacy, School of Pharmacy, Shujitsu University 1-6-1 Nishigawara, Okayama 703-8516, Japan 2Department of Environmental Sciences, Faculty of Science, Ibaraki University Mito, Ibaraki 310-8512, Japan 3Institute of Advanced Energy, Kyoto University Gokasho, Uji 611-0011, Japan 4Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, National Institute of Health Research Triangle Park, NC 27709, USA

*To whom correspondence should be addressed. Tel: +81 82 424 7457; Fax: +81 82 424 7457; Email: ideh{at}hiroshima-u.ac.jp

Received January 26, 2005. Revised March 29, 2005. Accepted March 29, 2005.

Nitric oxide (NO) induces deamination of guanine, yielding xanthine and oxanine (Oxa). Furthermore, Oxa reacts with polyamines and DNA binding proteins to form cross-link adducts. Thus, it is of interest how these lesions are processed by DNA repair enzymes in view of the genotoxic mechanism of NO. In the present study, we have examined the repair capacity for Oxa and Oxa–spermine cross-link adducts (Oxa–Sp) of enzymes involved in base excision repair (BER) and nucleotide excision repair (NER) to delineate the repair mechanism of nitrosative damage to guanine. Oligonucleotide substrates containing Oxa and Oxa–Sp were incubated with purified BER and NER enzymes or cell-free extracts (CFEs), and the damage-excising or DNA-incising activity was compared with that for control (physiological) substrates. The Oxa-excising activities of Escherichia coli and human DNA glycosylases and HeLa CFEs were 0.2–9% relative to control substrates, implying poor processing of Oxa by BER. In contrast, DNA containing Oxa–Sp was incised efficiently by UvrABC nuclease and SOS-induced E.coli CFEs, suggesting a role of NER in ameliorating genotoxic effects associated with nitrosative stress. Analyses of the activity of CFEs from NER-proficient and NER-deficient human cells on Oxa–Sp DNA confirmed further the involvement of NER in the repair of nitrosative DNA damage.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
S. P. Pack, N. K. Kamisetty, M. Nonogawa, K. C. Devarayapalli, K. Ohtani, K. Yamada, Y. Yoshida, T. Kodaki, and K. Makino
Direct immobilization of DNA oligomers onto the amine-functionalized glass surface for DNA microarray fabrication through the activation-free reaction of oxanine
Nucleic Acids Res., September 27, 2007; 35(17): e110 - e110.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. T. Reardon and A. Sancar
Repair of DNA-polypeptide crosslinks by human excision nuclease.
PNAS, March 14, 2006; 103(11): 4056 - 4061.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. P. Pack, M. Nonogawa, T. Kodaki, and K. Makino
Chemical synthesis and thermodynamic characterization of oxanine-containing oligodeoxynucleotides
Nucleic Acids Res., October 11, 2005; 33(18): 5771 - 5780.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.