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Nucleic Acids Research 2005 33(Database Issue):D403-D406; doi:10.1093/nar/gki099
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Nucleic Acids Research, 2005, Vol. 33, Database issue D403-D406
© 2005, the authors
Nucleic Acids Research, Vol. 33, Database issue © Oxford University Press 2005; all rights reserved

SilkSatDb: a microsatellite database of the silkworm, Bombyx mori

M. D. Prasad, M. Muthulakshmi, K. P. Arunkumar, M. Madhu, V. B. Sreenu1, V. Pavithra1, B. Bose1, H. A. Nagarajaram1, K. Mita2, T. Shimada3 and J. Nagaraju*

Laboratories of Molecular Genetics and 1 Computational Biology and Bioinformatics Facility (EMBnet India Node), Centre for DNA Fingerprinting and Diagnostics, ECIL Road, Nacharam, Hyderabad 500076, India, 2 Insect Genome, National Institute of Agrobiological Sciences, Owashi 1-2, Tsukuba, Ibaraki 305-8634, Japan and 3 Laboratory of Insect Genetics and Bioscience, Department of Agricultural and Environmental Biology, University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan

* To whom correspondence should be addressed. Tel: +91 40 27171427; Fax: +91 40 27155610; Email: jnagaraju{at}cdfd.org.in

Received May 27, 2004; Revised and Accepted October 14, 2004

The SilkSatDb (silkmoth microsatellite database) (http://www.cdfd.org.in/silksatdb) is a relational database of microsatellites extracted from the available expressed sequence tags and whole genome shotgun sequences of the silkmoth, Bombyx mori. The database has been rendered with a simple and robust web-based search facility, developed using PHP. The SilkSatDb also stores information on primers developed and validated in the laboratory. Users can retrieve information on the microsatellite and the protocols used, along with informative figures and polymorphism status of those microsatellites. In addition, the interface is coupled with Autoprimer, a primer-designing program, using which users can design primers for the loci of interest.

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