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Nucleic Acids Research 2005 33(Database Issue):D550-D552; doi:10.1093/nar/gki085
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Nucleic Acids Research, 2005, Vol. 33, Database issue D550-D552
© 2005, the authors
Nucleic Acids Research, Vol. 33, Database issue © Oxford University Press 2005; all rights reserved

5'SAGE: 5'-end Serial Analysis of Gene Expression database

Yasuhiro Kasai1,4, Shin-ichi Hashimoto3, Tomoyuki Yamada1, Jun Sese1, Sumio Sugano2, Kouji Matsushima3 and Shinichi Morishita1,*

1 Department of Computational Biology and 2 Department of Medical Genome Sciences, Graduate School of Frontier Science, The University of Tokyo, 5-1-5 Kashinoha, Kashiwa City, Chiba 277-8562, Japan, 3 Department of Molecular Preventive Medicine, School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan and 4 Hitachi Software Engineering Co., Ltd, 1-1-45 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan

* To whom correspondence should be addressed. Tel: +81 47 136 3984; Fax: +81 47 136 3977; Email: moris{at}k.u-tokyo.ac.jp

Received August 13, 2004; Revised and Accepted October 12, 2004

To comprehensively identify transcription start sites and the frequencies of individual mRNAs in human cell libraries, a method of 5' end Serial Analysis of Gene Expression (SAGE) was developed recently, which makes it possible to collect a large amount of start site information, and subsequently, we have established a related database server called 5'SAGE. This database displays the observed frequencies of individual 5' end SAGE tags and previously unknown transcription start sites in the promoter regions, introns and intergenic regions of known genes. 5'SAGE will be useful for analyzing promoter regions and start site variation in different tissues, and is freely available at http://5sage.gi.k.u-tokyo.ac.jp/.

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