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Nucleic Acids Research 2005 33(Web Server Issue):W669-W672; doi:10.1093/nar/gki436
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© The Author 2005. Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions{at}oupjournals.org

Article

TargetIdentifier: a webserver for identifying full-length cDNAs from EST sequences

Xiang Jia Min1,*, Gregory Butler1,2, Reginald Storms1,3 and Adrian Tsang1,3

1Centre for Structural and Functional Genomics, Concordia University Montreal, Quebec H4B 1R6, Canada 2Department of Computer Science, Concordia University Montreal, Quebec H4B 1R6, Canada 3Department of Biology, Concordia University Montreal, Quebec H4B 1R6, Canada

*To whom correspondence should be addressed. Tel: +1 514 848 2424, ext. 5791; Fax: +1 514 848 4504; Email: jack{at}gene.concordia.ca

Received February 13, 2005. Revised March 25, 2005. Accepted March 25, 2005.

TargetIdentifier is a webserver that identifies full-length cDNA sequences from the expressed sequence tag (EST)-derived contig and singleton data. To accomplish this TargetIdentifier uses BLASTX alignments as a guide to locate protein coding regions and potential start and stop codons. This information is then used to determine whether the EST-derived sequences include their translation start codons. The algorithm also uses the BLASTX output to assign putative functions to the query sequences. The server is available at https://fungalgenome.concordia.ca/tools/TargetIdentifier.html.


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