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Nucleic Acids Research 2006 34(10):2847-2852; doi:10.1093/nar/gkl355
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Published online 24 May 2006

© The Author 2006. Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org

Article

Human Rad51 protein displays enhanced homologous pairing of DNA sequences resembling those at genetically unstable loci

Erica M. Seitz and Stephen C. Kowalczykowski*

Sections of Microbiology and of Molecular and Cellular Biology, Center for Genetics and Development, Microbiology Graduate Group, University of California Davis, CA 95616-8665, USA

*To whom correspondence should be addressed. Tel: +1 530 752 5938; Fax: +1 530 752 5939; Email: sckowalczykowski{at}ucdavis.edu

Received March 7, 2006. Revised April 3, 2006. Accepted April 20, 2006.

DNA strand exchange, the central step of homologous recombination, is considered to occur approximately independently of DNA sequence content. However, certain prokaryotic and eukaryotic genomic loci display either an enhanced or reduced frequency of genetic exchange. Here we show that the Homo sapiens DNA strand exchange protein, HsRad51, shows a preference for binding to single-stranded DNA sequences primarily rich in G-residues and poor in A- and C-residues, and that these DNA sequences manifest enhanced HsRad51 protein-dependent homologous pairing. Both of these properties are common to all DNA strand exchange proteins examined thus far. These preferred DNA pairing sequences resemble those found at genetic loci in human cells that cause genomic instability and lead to genetic diseases.


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