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Nucleic Acids Research 2006 34(10):e75; doi:10.1093/nar/gkl364
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Published online 21 June 2006

© 2006 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commerical use, distribution, and reproduction in any medium, provided the original work is properly cited.

Methods Online

Electrochemical detection of mismatched DNA using a MutS probe

Minseon Cho, Sohyun Lee, Se-Young Han, Jin-Young Park, Md Aminur Rahman1, Yoon-Bo Shim1,* and Changill Ban*

Department of Chemistry, Pohang University of Science and Technology Pohang, Gyungbuk, 790-784, South Korea 1 Department of Chemistry, Pusan National University Busan, 609-735, South Korea

*To whom correspondence should be addressed. Tel: +82 54 279 2127; Fax: +82 54 279 3399; Email: ciban{at}postech.ac.kr

Received November 12, 2005. Revised November 20, 2005. Accepted April 25, 2006.

A direct and label-free electrochemical biosensor for the detection of the protein–mismatched DNA interaction was designed using immobilized N-terminal histidine tagged Escherichia coli. MutS on a Ni-NTA coated Au electrode. General electrochemical methods, cyclic voltammetry (CV), electrochemical quartz crystal microbalance (EQCM) and impedance spectroscopy, were used to ascertain the binding affinity of mismatched DNAs to the MutS probe. The direct results of CV and impedance clearly reveal that the interaction of MutS with the CC heteroduplex was much stronger than that with AT homoduplex, which was not differentiated in previous results (GT > CT > CC {approx} AT) of a gel mobility shift assay. The EQCM technique was also able to quantitatively analyze MutS affinity to heteroduplexes.

*Correspondence may also be addressed to Yoon-Bo Shim. Tel: +82 51 510 2244; Fax: +82 51 516 7421; Email: ybshim{at}pusan.ac.kr


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