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Nucleic Acids Research Advance Access originally published online on August 16, 2006
Nucleic Acids Research 2006 34(14):4060-4068; doi:10.1093/nar/gkl577
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Nucleic Acids Research, 2006, Vol. 34, No. 14 4060-4068
© 2006 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Molecular Biology

Isolation of a single-stranded DNA-binding protein from the methylotrophic yeast, Pichia pastoris and its identification as zeta crystallin

Balla Venkata Kranthi, Natarajan Balasubramanian and Pundi N. Rangarajan*

Department of Biochemistry, Indian Institute of Science Bangalore 560012, India

*To whom correspondence should be addressed. Tel/Fax: +91 80 2360492; Email: pnr{at}biochem.iisc.ernet.in

Received June 12, 2006. Revised July 20, 2006. Accepted July 24, 2006.

A single-stranded DNA (ssDNA)-binding protein (SSB) that binds to specific upstream sequences of alcohol oxidase (AOX1) promoter of the methylotrophic yeast Pichia pastoris has been isolated and identified as zeta crystallin (ZTA1). The cDNA encoding P.pastoris ZTA1 (PpZTA1) was cloned into an Escherichia coli expression vector, the recombinant PpZTA1 was expressed and purified from E.coli cell lysates. The DNA-binding properties of recombinant PpZTA1 are identical to those of the SSB present in P.pastoris cell lysates. PpZTA1 binds to ssDNA sequences >24 nt and its DNA-binding activity is abolished by NADPH. This is the first report on the characterization of DNA-binding properties of a yeast ZTA1.


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