Nucleic Acids Research Advance Access originally published online on September 8, 2006
Nucleic Acids Research 2006 34(16):4593-4608; doi:10.1093/nar/gkl603
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Nucleic Acids Research, 2006, Vol. 34, No. 16 4593-4608
© 2006 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Molecular Biology |
NOP132 is required for proper nucleolus localization of DEAD-box RNA helicase DDX47
Department of Molecular Biology, Graduate School of Medical Science, Kyushu University 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan 1 Department of Applied Biological Science, Tokyo University of Agriculture and Technology 3-5-8 Saiwai-cho, fuchu, Tokyo 183-8509, Japan
*To whom correspondence should be addressed. Tel: +81 92 642 6177; Fax: +81 92 642 6183; Email: sekigu{at}molbiol.med.kyushu-u.ac.jp
Received April 5, 2006. Revised July 11, 2006. Accepted August 3, 2006.
Previously, we described a novel nucleolar protein, NOP132, which interacts with the small GTP binding protein RRAG A. To elucidate the function of NOP132 in the nucleolus, we identified proteins that interact with NOP132 using mass spectrometric methods. NOP132 associated mainly with proteins involved in ribosome biogenesis and RNA metabolism, including the DEAD-box RNA helicase protein, DDX47, whose yeast homolog is Rrp3, which has roles in pre-rRNA processing. Immunoprecipitation of FLAG-tagged DDX47 co-precipitated rRNA precursors, as well as a number of proteins that are probably involved in ribosome biogenesis, implying that DDX47 plays a role in pre-rRNA processing. Introduction of NOP132 small interfering RNAs induced a ring-like localization of DDX47 in the nucleolus, suggesting that NOP132 is required for the appropriate localization of DDX47 within the nucleolus. We propose that NOP132 functions in the recruitment of pre-rRNA processing proteins, including DDX47, to the region where rRNA is transcribed within the nucleolus.
Present addresses: Toshiya Hayano, Department of Bioscience and Bioinformatics, College of Information Science and Engineering, Ritsumeikan University, 1-1-1 Nojihigashi, Kusatsu 525-8577, Japan
Mitsuaki Yanagida, Institute for Environmental and Gender Specific Medicine, Juntendo University, Graduate School of Medicine, 2-1-1, Tomioka, Urayasu, Chiba 279-0021, Japan
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