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Nucleic Acids Research Advance Access originally published online on September 26, 2006
Nucleic Acids Research 2006 34(18):5238-5246; doi:10.1093/nar/gkl688
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Nucleic Acids Research, 2006, Vol. 34, No. 18 5238-5246
© 2006 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Genomics

The proteins encoded by the pogo-like Lemi1 element bind the TIRs and subterminal repeated motifs of the Arabidopsis Emigrant MITE: consequences for the transposition mechanism of MITEs

Céline Loot, Néstor Santiago, Alicia Sanz and Josep M. Casacuberta*

Departament de Genètica Molecular, Laboratori de Genètica Molecular Vegetal CSIC-IRTA Jordi Girona 18, 08034 Barcelona, Spain

*To whom correspondence should be addressed. Tel: +34 93 4006142; Fax: +34 93 2045904; Email: jcsgmp{at}ibmb.csic.es

Received May 22, 2006. Revised August 10, 2006. Accepted September 7, 2006.

MITEs (miniature inverted-repeated transposable elements) are a particular class of defective DNA transposons usually present within genomes as high copy number populations of highly homogeneous elements. Although an active MITE, the mPing element, has recently been characterized in rice, the transposition mechanism of MITEs remains unknown. It has been proposed that transposases of related transposons could mobilize MITEs in trans. Moreover, it has also been proposed that the presence of conserved terminal inverted-repeated (TIR) sequences could be the only requirement of MITEs for mobilization, allowing divergent or unrelated elements to be mobilized by a particular transposase. We present here evidence for a recent mobility of the Arabidopsis Emigrant MITE and we report on the capacity of the proteins encoded by the related Lemi1 transposon, a pogo-related element, to specifically bind Emigrant elements. This suggests that Lemi1 could mobilize Emigrant elements and makes the Lemi1/Emigrant couple an ideal system to study the transposition mechanism of MITEs. Our results show that Lemi1 proteins bind Emigrant TIRs but also bind cooperatively to subterminal repeated motifs. The requirement of internal sequences for the formation of proper DNA/protein structure could affect the capacity of divergent MITEs to be mobilized by distantly related transposases.


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[Abstract] [Full Text] [PDF]


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