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Nucleic Acids Research 2006 34(4):1092-1101; doi:10.1093/nar/gkj507
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Published online 14 February 2006

© The Author 2006. Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions{at}oxfordjournals.org

Article

Role of the silkworm argonaute2 homolog gene in double-strand break repair of extrachromosomal DNA

Haruna Tsukioka, Masateru Takahashi, Hiroaki Mon, Kazuhiro Okano1, Kazuei Mita2, Toru Shimada3, Jae Man Lee, Yutaka Kawaguchi, Katsumi Koga and Takahiro Kusakabe*

Laboratory of Silkworm Sciences, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences 6-10-1 Hakozaki, Fukuoka 812-8581, Japan 1Laboratory of Molecular Entomology and Baculovirology, The Institute of Physical and Chemical Research (RIKEN) Hirosawa 2-1, Wako, Saitama 351-0198, Japan 2Laboratory of Insect Genome, National Institute of Agrobiological Sciences Owashi 1-2, Tsukuba, Ibaraki 305-8634, Japan 3Department of Agricultural and Environmental Biology, University of Tokyo Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan

*To whom correspondence should be addressed. Tel: +81 92 642 2842; Fax: +81 92 642 2842; Email: kusakabe{at}agr.kyushu-u.ac.jp

Received January 25, 2006. Accepted January 27, 2006.

The argonaute protein family provides central components for RNA interference (RNAi) and related phenomena in a wide variety of organisms. Here, we isolated, from a Bombyx mori cell, a cDNA clone named BmAGO2, which is homologous to Drosophila ARGONAUTE2, the gene encoding a repressive factor for the recombination repair of extrachromosomal double-strand breaks (DSBs). RNAi-mediated silencing of the BmAGO2 sequence markedly increased homologous recombination (HR) repair of DSBs in episomal DNA, but had no effect on that in chromosomes. Moreover, we found that RNAi for BmAGO2 enhanced the integration of linearized DNA into a silkworm chromosome via HR. These results suggested that BmAgo2 protein plays an indispensable role in the repression of extrachromosomal DSB repair.

Present address: Kazuhiro Okano, Department of Microbiology, Nash Hall 220, Oregon State University, Corvallis, OR 97331-3804, USA

Katsumi Koga, Department of Biological Substances and Life Science, Kyushu Kyoritsu University, Kitakyushu 807-8585, Japan

The authors wish it to be known that, in their opinion, the first three authors should be regarded as joint First Authors


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